Cu2+配位逆流色谱分离纯化抗霉素同系物

摘要

Copper chelated high speed countercurrent chromatography(Cu2+-chelated-HSCCC)is applied for the separation and purification of the crude extractions of antimycin fermentation liquor, by use of n-hexane/methanol/ acetate ester/water (5:3:2:2, V/V) as the respective two-phase solvent systems. The lower phase was used as the mobile phase and upper phase as stationary phase. The purity of separated fractions was analyzed by high performance liquid chromatography (HPLC). Experiment data shows the nine separated fractions could be abtained respectively at a high purity (>96%)and a recovery rate (>60%) just through one-step HSCCC .%应用Cu2+配位逆流色谱对抗霉素发酵粗提物进行分离纯化研究,选择正己烷/甲醇/乙酸乙酯/水(5:3:2:2,V/V)为两相体系,以上相为固定相,下相为流动相进行分离纯化,用高效液相色谱仪测定单组份纯度.实验结果表明Cu2+配位逆流色谱可将抗霉素粗品分离纯化,得到9个纯度96％以上的抗霉素单组份,收率达60％以上.