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首页> 外文期刊>Journal of separation science. >Application of coordination agent in high‐speed counter‐current chromatography for the preparative separation and isolation ginkgolic acids from the sarcotesta of Ginkgo bilobaGinkgo biloba L
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Application of coordination agent in high‐speed counter‐current chromatography for the preparative separation and isolation ginkgolic acids from the sarcotesta of Ginkgo bilobaGinkgo biloba L

机译：在高速逆流色谱中施用配位逆流色谱法从银杏的Sarcotesta中的制备分离和分离甘油酸银杏BILOBA L.

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Abstract > An efficient coordination high‐speed counter‐current chromatography method for the preparative separation of ginkgolic acids from the sarcotesta of Ginkgo biloba L was developed. The type, concentration, and mechanism of the coordination agent were investigated. Following the use of four types of metal salts including silver nitrate, copper chloride, ferric chloride, and aluminium nitrate, n ‐heptane/ethyl acetate/methanol/acetic acid 5:4:1:1, v/v with 0.20?mol/L silver nitrate as the coordination agent was chosen as the optimum two‐phase solvent system. Five main ginkgolic acids including C13:0, C15:0, C15:1, C17:1, and C17:2 were successfully separated with purities greater than 98%. The sample loading was 500?mg, the flow‐rate was 2.0?mL/min, rotation speed was 800 rpm and temperature was 20°C. The structures of the separated ginkgolic acids were identified by comparison with standard samples and electrospray ionization mass spectrometry. The introduction of coordination chemistry in high‐speed counter‐current chromatography is novel and effective for the preparative separation and isolation of ginkgolic acids from the sarcotesta of Ginkgo biloba L and could also be applied to separate compounds which form coordination bonds in other complex natural products. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >一种高效的协调高速逆流色谱方法，用于甘油酸的制备分离SARCOTESTA的银杏BILOBA </ i> L是开发的。研究了协调剂的类型，浓度和机理。在使用四种类型的金属盐之后，包括硝酸银，氯化铜，氯化铁和硝酸铝， N </ I> - 乙酸乙酯/乙酸乙酯/甲醇/乙酸5：4：1：1，V / v为0.20？mol / l硝酸银，因为选择配角作为最佳的两相溶剂体系。包括C13：0，C15：0，C15：1，C17：1和C17：2在内的五个主要的甘油酸成功地分离出大于98％的纯度。样品负载为500〜Mg，流速为2.0？ml / min，转速为800rpm，温度为20℃。通过与标准样品和电喷雾电离质谱法进行比较来鉴定分离的甘油酸的结构。高速逆流色谱中的协调化学的引入是新颖的，并且对于来自银杏叶的Sarcotesta的制备分离和分离甘油酸的分离，并且也可以应用于不同的组合物在其他复杂的天然产品中的协调键。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2018年第23期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhu Heng&option=202" target="_blank" rel="nofollow">Zhu Heng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jiang Hailong&option=202" target="_blank" rel="nofollow">Jiang Hailong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yu Jinqian&option=202" target="_blank" rel="nofollow">Yu Jinqian;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Song Xiangyun&option=202" target="_blank" rel="nofollow">Song Xiangyun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhao Hengqiang&option=202" target="_blank" rel="nofollow">Zhao Hengqiang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Jingchao&option=202" target="_blank" rel="nofollow">Li Jingchao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Geng Yanling&option=202" target="_blank" rel="nofollow">Geng Yanling;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Daijie&option=202" target="_blank" rel="nofollow">Wang Daijie;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Key Laboratory of TCM Quality ControlQilu University of Technology (Shandong Academy of Sciences)Jinan P. R. China;</p> <p>Key Laboratory of TCM Quality ControlQilu University of Technology (Shandong Academy of Sciences)Jinan P. R. China;</p> <p>Key Laboratory of TCM Quality ControlQilu University of Technology (Shandong Academy of Sciences)Jinan P. R. China;</p> <p>Key Laboratory of TCM Quality ControlQilu University of Technology (Shandong Academy of Sciences)Jinan P. R. China;</p> <p>Key Laboratory of TCM Quality ControlQilu University of Technology (Shandong Academy of Sciences)Jinan P. R. China;</p> <p>Key Laboratory of TCM Quality ControlQilu University of Technology (Shandong Academy of Sciences)Jinan P. R. China;</p> <p>Key Laboratory of TCM Quality ControlQilu University of Technology (Shandong Academy of Sciences)Jinan P. R. China;</p> <p>Key Laboratory of TCM Quality ControlQilu University of Technology (Shandong Academy of Sciences)Jinan P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=coordination chemistry&option=203" rel="nofollow">coordination chemistry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ginkgo biloba L.&option=203" rel="nofollow">Ginkgo biloba L.;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ginkgolic acids&option=203" rel="nofollow">ginkgolic acids;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high‐speed counter‐current chromatography&option=203" rel="nofollow">high‐speed counter‐current chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=silver nitrate&option=203" rel="nofollow">silver nitrate;</a> </p> <div class="translation"> 机译：协调化学;银杏酸;甘油酸;高速逆流色谱;银硝酸盐; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025846151.html">Application of coordination agent in high‐speed counter‐current 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=殷梦舟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 殷梦舟</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘莹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘莹</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张莉佳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张莉佳</a> <span> <a href="/journal-cn-9417/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 食品科学 </a> </span> <span> . 2020</span><span>,第016期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-traditional-patent-medicine_thesis/0201279233948.html">高速逆流色谱法分离制备厚朴中magnoloside A</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=胡小龙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 胡小龙</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=姜峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,姜峰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张敏&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张敏</a> <span> <a href="/journal-cn-5710/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中成药 </a> </span> <span> . 2020</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-12421_thesis/02022638725.html">硅胶柱色谱结合高速逆流色谱法分离制备丹参中丹参酮</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=蓝天凤&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 蓝天凤</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=于宗渊&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,于宗渊</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王岱杰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王岱杰</a> <span> <a href="/conference-cn-12421/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2010年全国中药学术研讨会 </a> <span> <span> . 2010</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/02031373624.html">高速逆流色谱法分离纯化藏药湿生扁蕾中的有效成分</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=金盼盼&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 金盼盼</a> <span> . 2012</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201038740.html">高速逆流色谱法一步制备分离鸢尾中鸢尾苷元的方法</a> <b>[P]</b> . <span> 中国专利： CN101906091B </span> <span> . 2012.10.17</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120106116704.html">高速逆流色谱法一步制备分离鸢尾中鸢尾苷元的方法</a> <b>[P]</b> . <span> 中国专利： CN101906091A </span> <span> . 2010-12-08</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130422975192.html">The process of extracting insecticides from ginkgolic acid solution, including liquid and liquid extractions, to obtain an average polar fraction containing two-thirds of Ginkgo and pesticides, as well as a high polar fraction containing glucose from Ginkgo biloba. Default third person in Ginza</a> <b>[P]</b> . <span> 外国专利： <!-- --> CL2011002474A1 </span> <span> . 2012-03-23</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从银杏酸溶液中提取杀虫剂的过程，包括液体提取和液体提取，以获取平均含有三分之二的银杏和农药的极性馏分，以及含有来自银杏的葡萄糖的高极性馏分。银座的预设第三人称 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130436621041.html">Preparing extract from Ginkgo biloba having reduced content of 4'-O-methyl pyridoxine and/or biflavones for treating e.g. dementia involves preparing Ginkgo extract solution; applying solution to adsorber resin or an acidic ion exchanger</a> <b>[P]</b> . <span> 外国专利： <!-- 德国专利： --> DE102006019861A1 </span> <span> . 2006-11-09</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从银杏制备具有降低的4'-O-甲基吡ido醇和/或双黄酮含量的银杏提取物用于治疗例如痴呆症涉及制备银杏提取液;将溶液施加到吸附树脂或酸性离子交换剂上 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130419402896.html">METHOD FOR PREPARING GINSENOSIDE Rg3 USING RECOMBINANT BETA-GLUCOSIDASE GENE-DERIVED ENZYME AND HIGH SPEED COUNTER CURRENT CHROMATOGRAPHY AFTER MEDIUM PRESSURE LIQUID CHROMATOGRAPHY PRETREATMENT</a> <b>[P]</b> . <span> 外国专利： <!-- 韩国专利： --> KR20130110658A </span> <span> . 2013-10-10</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：中压液相色谱预处理后使用重组β-葡糖苷酶基因衍生酶和高速反流色谱法制备人参皂苷Rg3的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" 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