鸭腔上囊胚胎及胚后发育期凋亡相关蛋白的免疫组化分析

摘要

研究天府肉鸭腔上囊胚胎及胚后发育期Bcl-2、Bax、Fas、FasL蛋白的表达.将20只天府肉鸭分为4组,即24天胚龄(E24),胚后3、8、29周龄(P3、P8、P29),采用免疫组化技术.结果显示,Bcl-2、Bax、Fas、FasL在各组滤泡淋巴细胞中均有表达,FasL还表达于滤泡间上皮.滤泡皮质和髓质淋巴细胞Bcl-2阳性率呈递减的变化趋势(髓质P3～8除外);皮质和髓质淋巴细胞Bax阳性率在E24～P8恒定,P29上升;皮质和髓质淋巴细胞Bcl-2/Bax值呈下降趋势,但皮质在P3～8恒定,髓质在P3～8上升;滤泡皮质和髓质淋巴细胞Fas阳性率在E24～P3 上升,P3～8下降,P8～29上升;皮质和髓质淋巴细胞FasL阳性率变化规律与Fas相似,但皮质在P3～8恒定.滤泡皮质淋巴细胞Bcl-2阳性率及Bcl-2/Bax值在E24～P8明显高于髓质,在P29则显著低于髓质;皮质淋巴细胞Bax阳性率在E24～P8与髓质无显著差异,在P29则明显高于髓质;皮质淋巴细胞Fas和FasL阳性率在E24～P8明显低于髓质,在P29则显著高于髓质.结果提示,Bcl-2、Bax、Fas、FasL是鸭腔上囊胚胎及胚后发育期淋巴细胞凋亡的重要调节物.%This experiment was conducted to study the expression of Bcl-2 , Bax, Fas and FasL proteins during the embryonic and post embryonic development of the Tianfu duck bursa of Fabricius. 20 Tianfu ducks were divided into 4 groups as follows: on embryonic day 24 (E24), and postembryonic week 3, 8 and 29'(P3, P8, P29). Immunohistochemisty technique was used in this study. The results showed that Bcl-2, Bax, Fas and FasL were expressed in the follicle lymphocytes, and FasL was also displayed in the interfollicular epithelia. The Bcl-2 positive ratios of lymphocytes in the follicle cortex and medulla progressively decreased (except P3-8 in the medulla). The Bax positive ratios of lymphocytes remained stable from E24-P8, but dramatically rose on P29. The values of Bcl-2/Bax of lymphocytes showed a decreased tendency; however, they remained unchanged in the cortex and rose in the medulla from P3-8, respectively. The Fas positive ratios of lymphocytes increased from E24-P3 and P8-29, but decreased from P3-8. The changing pattern of FasL positive ratios of lymphocytes was similar to that of Fas, however, it remained stable from P3-8. Furthermore, the Bcl-2 positive ratios and the values of Bcl-2/Bax of lymphocytes in the follicle cortex were significantly higher from E24P8 and lower on P29 than those in the follicle medulla, respectively. The Bax positive ratios of lymphocytes in the follicle cortex were not significant different from those in the medulla from E24-P8, but higher than those in the medulla on P29. The Fas and FasL positive ratios of lymphocytes in the follicle cortex were significantly lower from E24-P8 and higher on P29 than those in the medulla. This study indicated that Bcl-2, Bax, Fas and FasL proteins were important modulators for the lymphocyte apoptosis of the duck bursa of Fabricius during the embryonic and post embryonic development.