This study aimed to research characteristics of poultry embryo amniotic epithelial cells (AECs) which were isolated, cultured, identified and induced to differentiate to different meso-dermal cells. AECs were isolated from Beijing-You chicken fetal membranes using enzymes digestion, immunofluorescence and RT-PCR were used to identify Nanog, Oct-4, Sox-2, CK19 and Vimentin. The multi-lineage differentiation potential of AECs was investigated, which were induced to differentiate into osteoblasts, adipocytes and pancreatic-islet like cells cells. The result showed that chicken AECs not only expressed the special markers of stem cells, but were induced to differentiate into osteoblasts, adipocytes and pancreatic-islet like ceils successfully. To sum up, the AECs isolated from Beijing-You chicken fetal membranes have a high reproductive capacity and plasticity in vitro , and can be used as seed cells for the preservation of endangered species and for clinical treatment. The results can provide the theoretical basis for clinical treatment using AECs.%旨在研究禽类胚胎羊膜上皮细胞的分离、培养、鉴定及向不同胚层细胞诱导分化等的生物学特性.本研究使用酶消化法从北京油鸡胎膜中分离羊膜上皮细胞,通过免疫荧光和RT-PCR方法鉴定羊膜上皮细胞标志物(Nanog、Oct-4、Sox-2、CK19和Vimentin),并诱导其向成骨细胞、脂肪细胞和胰岛样细胞诱导分化,检测其多向分化潜能.结果表明,酶消化法获得的鸡羊膜上皮细胞不仅表达干细胞特有的标志,而且成功诱导分化为成骨细胞、脂肪细胞和胰岛样细胞.综上所述,北京油鸡羊膜上皮细胞在体外具有较强的自我更新能力及可塑性,可作为种子细胞进行濒危物种的保存,并为日后利用羊膜上皮细胞进行临床治疗提供一定的理论依据,同时也可为油鸡基因的改良提供模型.
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