(—)-HCA对鸡胚原代肝细胞增殖和线粒体功能的影响

摘要

The present study aimed to investigate the effects of (—)-Hydroxycitric acid (HCA) on cells proliferation,mitochondrial ultrastructure and function in primary chicken hepatocytes.The cells were treated with 0,1,10 and 50μmol · L-1 (—)-HCA and then detected the factors related with cells proliferation and mitochondria function.The results showed that (—)-HCA treatment promoted the cell proliferation in primary chicken hepatocytes.Tenμmol · L-1 (—)-HCA treatment significantly increased the S phase and G2/M phase cell population (P＜0.01),meanwhile it increased the cyclin mRNA expression level when compared to control group.No significantly changes were observed on the mitochondrial morphology,quantity and membrane potential in primary chicken hepatocytes following 1-50 μtmol · L-1 (—)-HCA treatment (P ＞ 0.05),while (—)-HCA treatment significantly increased the activities of succinate dehydrogenase and citrate synthase in primary chicken hepatocytes (P＜0.05).These results indicated that (—)-HCA treatment promote the primary chicken hepatocytes proliferation by increasing cyclin mRNA expression,whereas it increase the energy metabolism by enhancing the activities of succinate dehydrogenase and citrate synthase in primary chicken hepatocytes.%试验旨在研究(—)-HCA对鸡胚原代肝细胞增殖和线粒体超微结构及功能的影响.笔者选用终浓度为0、1、10和50 μmol·L-1的(—)-HCA处理鸡胚原代肝细胞后收集细胞,检测细胞增殖及线粒体功能相关指标.结果表明,(—)-HCA处理可促进鸡胚原代肝细胞增殖,10 μmol·L-1(—)-HCA处理可极显著升高S期和G2/M期细胞比例(P＜0.01),并提高周期相关因子的mRNA转录.1～50 μmol·L-1(—)-HCA处理对鸡胚原代肝细胞线粒体形态、数量和膜电位均无显著影响(P＞0.05),但(—)-HCA处理可显著升高原代鸡胚肝细胞线粒体中柠檬酸合酶和琥珀酸脱氢酶活性(P＜0.05).结果提示,(—)-HCA处理可通过调节周期相关因子的表达而促进鸡胚原代肝细胞的增殖,同时其可通过加速三羧酸循环进而增强细胞的能量代谢水平.