谷胱甘肽对奥尼罗非鱼原代肝细胞增殖及生化功能的影响

摘要

[目的]研究谷胱甘肽(Glutathione,GSH)对奥尼罗非鱼Oreochromis niloticus×O.aureus原代肝细胞增殖和生化功能的影响,探究谷胱甘肽的促生长作用机制.[方法]用添加ψ为10％胎牛血清和未添加血清的培养液培养奥尼罗非鱼原代肝细胞48 h,随后在培养液中分别加GSH至0、30、100、300、900mg·L-1,每个水平设6个重复,培养24、48和72 h后测定奥尼罗非鱼原代肝细胞增殖情况及培养液中胰岛素样生长因子、白蛋白、过氧化氢含量和谷草转氨酶、γ-谷氨酰转肽酶活性.[结果]谷胱甘肽显著促进了奥尼罗非鱼原代肝细胞增殖,提高了培养液上清中胰岛素样生长因子、白蛋白的含量和γ-谷氨酰转肽酶活性,降低了培养液上清中过氧化氢含量和谷草转氨酶活性(P＜0.05).在无血清培养试验组中添加谷胱甘肽,对奥尼罗非鱼原代肝细胞增殖及生化指标的影响比添加血清培养试验组效果明显.[结论]谷胱甘肽能促进奥尼罗非鱼肝细胞增殖,且具有保护肝脏的作用.%[Objective] To investigate the effects of glutathione (GSH) on proliferation and biochemical function of primary hepatocyte in hybrid tilapia (Oreochromis niloticus× O.aureus).[Method] Tilapia primary hepatocytes were cultured in cell culture medium with 0 or 10％ fetal calf serum for 48 h.GSH was then added into the two kinds of cell culture medium to the final concentration of 0,30,100,300 or 900 mg·L-1 respectively.Each concentration group had six replicates.The proliferation status of the primary hepatocyte,contents of insulin-like growth factor 1 (IGF-1),albumin and H2O2,as well as glutamate-oxaloacetate transaminase (GOT) and γ-glutamyl transpeptidase (γ-GT) activities of tilapia were detected after cultured for 24,48 and 72 h,respectively.[Result] GSH significantly increased the proliferation of primary hepatocyte,the contents of IGF-1 and albumin,and γ-GT activity in supernatant,while reduced H2O2 content and GOT activity(P＜0.05).Such effects of GSH were more evident in the group without fetal calf serum compared to the group with 10％ fetal calf serum.[Conclusion] GSH could enhance the proliferation of primary hepatocyte and has the function of protecting liver in hybrid tilapia.