试验旨在研究油佐剂疫苗在鸡蛋和组织中残留规律.选用120只1日龄的京粉蛋鸡作为试验对象,每组设12个重复,每重复10只.按照免疫程序推荐剂量注射油佐剂疫苗,全部右侧翅肩肌注.在159、166、180、201、235、271 d测定鸡蛋中正烷烃和多环芳烃的残留量,同时在280、330、380、430 d测定右侧翅肩肌、左侧胸肌、右侧胸肌、左侧腿肌、右侧腿肌、肝、肾中正烷烃和多环芳烃的残留量.结果表明:矿物油中分子量较小的烷烃,如正十六烷、正十七烷、正十八烷,更易于被机体代谢,残留消除速率较快,也易于扩散到机体其他部位;矿物油进入机体后,在注射部位中残留量最大,通过体循环扩散到机体其他组织、器官中,在肝、肾中的残留量较大,且消除速率较慢;各个采样部位正烷烃和多环芳烃残留量随着时间的变化而降低.鸡蛋中未检测到多环芳烃.矿物油经过机体代谢进入鸡蛋中的量很少,不足以对鸡蛋质量安全造成影响;而它在鸡体内组织、器官中形成较长期的残留,特别是长链烷烃需要更长时间的代谢.%This experiment was conducted to study the residue dynamics of oil adjuvant in eggs and chicken.One hundred and twenty 1-day-old Jingfen layer chickens were raised.Every group was allocated into 12 replicates and 10 layer chickens per replicate.All the right wing shoulder muscle was injected in accordance with the recommended injection dose of oil adjuvant vaccine immune program.3 eggs were collected randomly from every replicate of group at 159,166,180,201,235,and 271 d to measure the residual of n-alkanes and PAHs.Six chickens were chosen randomly from group to slaughter at 280,330,380,430 d,collecting right wing shoulder muscle,left thorax muscle,right thorax muscle,left leg muscle,right leg muscle,liver and kidney to measure the residual of n-alkanes and PAHs.The results showed as follows:The alkanes with smaller molecular weight of mineral oil,such as n-hexadecane,n-heptadecane,n-octadecane,were easier to metabolize and transfer to other part of chicken.The amount of mineral oil in injection site was most,the residue amount of liver and kidney also more by general circulation,and residue elimination rate was slower.n-alkanes and PAHs in all samples were declined as time goes on.Then it had not detected PHAs in egg.The mineral oil transferred to eggs is too little,which has no effect on the egg quality.The mineral oil residue time in the chicken body is long,the long chain alkanes eliminated and migrated need more time to metabolized completely in the chicken.
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