槲皮素作为饲料添加剂的急性毒性和致突变性评价

摘要

This trial was conducted to evaluate quercetin safety as a feed additive. Acute toxicity test, Ames test, micronucleus test of bone marrow cells and sperm abnormality test were performed to evaluate quercetin safety. The results showed as follows: median lethal dosage (LD50) of quecertin > 10 g/kg BW; twice results of Ames test with and without liver microsomal polychlorinated biphenyl revulsive ( S9) were negative; the re-sult of micronucleus test of bone marrow cells was negative and there was no cytotoxic effect on experimental animals; the result of mouse sperm abnormality test showed that there was no significant difference in different quecertin treatments (P>0. 05) , and not in a dose-dependent manner, compared with the negative control group. Quercetin is safe as a feed additive.%本试验采用急性毒性试验、污染物致突变性检测(Ames试验)、小鼠骨髓细胞微核试验和小鼠精子畸形试验对槲皮素作为饲料添加剂的安全性进行评价.结果表明:槲皮素半数致死剂量(LD50)＞10 g/kg BW；Ames试验鼠伤寒沙门菌突变型各菌株在加与不加肝微粒体多氯联苯诱导剂(S9)情况下,经过2次试验结果均呈阴性；小鼠骨髓细胞微核试验结果为阴性,各槲皮素剂量组动物未见细胞毒性作用；小鼠精子畸形试验结果表明槲皮素剂量组与阴性对照组相比差异均不显著(P＞0.05),且没有剂量反应关系.试验结果提示,槲皮素是一种安全的饲料添加剂.