花鲈和西伯利亚鲟生长激素／类胰岛素样生长因子－Ⅰ轴相关基因全长 cDNA的克隆和序列分析

摘要

本研究采用反转录聚合酶链式反应（ RT⁃PCR）和cDNA末端快速扩增（ RACE）技术克隆花鲈（ Lateolabrax japonicus）和西伯利亚鲟（ Acipenser baerii Brandt）生长激素（ GH）和生长激素受体（ GHR ） cDNA 全长序列。结果显示：花鲈 GH （ GenBank 登录号 JQ995145） cDNA 全长949 bp，开放阅读框615 bp，编码204个氨基酸，预测分子质量为23．06 ku，与其他物种的相似性为36．0％～90．2％；花鲈GHRⅠ（ GenBank登录号JX402001） cDNA全长3070 bp，开放阅读框1911 bp，编码637个氨基酸，预测分子质量为70．79 ku，与其他物种的相似性为32．2％～86．2％；花鲈GHRⅡ（ GenBank登录号JQ995146） cDNA全长2926 bp，开放阅读框1749 bp，编码582个氨基酸，预测分子质量为64．42 ku，与其他物种的相似性为30．8％～81．4％。西伯利亚鲟GH（ GenBank登录号JX003684） cDNA全长999 bp，开放阅读框645 bp，编码214个氨基酸，预测分子质量为24．14 ku，与其他物种的相似性为38．3％～70．1％；西伯利亚鲟GHR（ GenBank登录号JX003685） cDNA全长2283 bp，开放阅读框1716 bp，编码571个氨基酸，预测分子质量为63．41 ku，与其他物种的相似性为39．5％～49．2％。花鲈和西伯利亚鲟GH和GHR cDNA全长序列的获得为进一步研究鱼类GH／类胰岛素样生长因子－Ⅰ（ IGF⁃Ⅰ）轴对生长发育的调控机制奠定了科学基础。%The reverse transcription⁃phosphatase chain reaction ( RT⁃PCR ) and rapid amplification of cDNA ends ( RACE) methods were used in this experiment to clone the full⁃length sequences of cDNAs for growth hormone ( GH) and GH receptor ( GHR) of Japanese seabass ( Lateolabrax japonicus) and Siberian sturgeon ( Acipenser baerii Brandt ) . The results showed as follows: the full⁃length GH cDNA of Japanese seabass ( GenBank accession No. JQ995145) was 949 bp, consisting of an open reading frame ( ORF) of 615 bp en⁃coding a protein of 204 amino acids with a theoretical molecular weight of 23. 06 ku, and shared 36. 0% to 90.2% similarity with other species. The full⁃length GHRⅠ cDNA of Japanese seabass ( GenBank accession No. JX402001) was 3 070 bp, consisting of an ORF of 1 911 bp encoding a protein of 637 amino acids with a theoretical molecular weight of 70.79 ku, and shared 32.2% to 86.2% similarity with other species. The full⁃length GHRⅡcDNA of Japanese seabass ( GenBank accession No. JQ995146) was 2 926 bp, consisting of an ORF of 1 749 bp encoding a protein of 582 amino acids with a theoretical molecular weight of 64.42 ku, and shared 30.8% to 81. 4% similarity with other species. The full⁃length GH cDNA of Siberian sturgeon ( Gen⁃Bank accession No. JX003684) was 999 bp, consisting of an ORF of 645 bp encoding a protein of 214 amino acids with a theoretical molecular weight of 24.14 ku, and shared 38.3% to 70.1% similarity with other spe⁃cies. The full⁃length GHR cDNA of Siberian sturgeon ( GenBank accession No. JX003685 ) was 2 238 bp, consisting of an ORF of 1 716 bp encoding a protein of 571 amino acids with a theoretical molecular weight of 63.41 ku, and shared 39. 5% to 49. 2% with other species. The full⁃length sequences of cDNAs for GH and GHR of Japanese seabass and Siberian sturgeon will provide a scientific basis for further study on the regulatory mechanism of GH/IGF⁃Ⅰ axis on growth and development of fish.