莱克多巴胺胶体金免疫层析法快速检测试纸条的研制

摘要

To develop a rapid and simple method to detect ractopamine （RAC） in pig urine, colloidal gold immunochromatography test strips were prepared. Colloidal gold particles prepared by sodium citrate reduction method were labeled with monoclonal antibody against ractopamine and sprayed onto the glass fiber. RAC-BSA conjugated antigen and goat anti-mouse IgG were bound onto a nitrocellulose membrane. Then, the sample pad, gold pad, nitrocellulose membrane and absorbent paper were assembled and cut into test strips. The detection limit of the test strips was 5ppb （ng/mL） ractopamine. The test could be completed within 8-10 min and the results could be read by naked eyes. There was no need for expensive equipment. The sensitivity, specificity, stability and reproducibility of the test strips met requirements for use in rapid testing and screening%为研制一种快速、简便的基于胶体金免疫层析法的试纸条,快速检测猪尿中莱克多巴胺（ractopamine,RAC药物残留）,采用柠檬酸钠还原法制备胶体金颗粒,标记莱克多巴胺单克隆抗体并喷于玻璃纤维上制备胶金垫,RAC-BSA偶联抗原和羊抗鼠IgG分别结合于硝酸纤维膜上,依次将样品垫、胶体金垫、硝酸纤维素膜和吸水纸组装并切割成莱克多巴胺胶体金免疫层析快速检测试纸条。试验结果表明该快速检测试纸条的检测限为5 ng/mL,可在8~10 min内完成测试,肉眼即可判断,无需其他检测设备。该试纸条灵敏度、特异性、稳定性和重复性均达到临床使用要求,适用于现场快速检测和筛选工作。