丘脑室旁核介导小鼠orexin能神经元的全麻促觉醒效应:采用光遗传学方法评价

摘要

目的 采用光遗传学方法 评价丘脑室旁核(PVT)介导小鼠促食欲素(orexin)能神经元的全麻促觉醒效应.方法健康雄性Hcrt-cre转基因小鼠20只,8～10周龄,体重20～25 g,采用随机数字表法分为4组(n=5):逆行病毒光敏感通道组(R组)、顺行病毒光敏感通道组(A组)、逆行病毒对照组(RC组)和顺行病毒对照组(AC组).利用光遗传学技术进行处理.采用1.0％异氟醚与纯氧1.0 L∕min诱导并维持麻醉,采用PowerLab脑电监测系统进行全程脑电监测,记录光刺激前1 min和光刺激期间的爆发性抑制率(BSR).结果 与RC组或光刺激前1 min比较,R组光刺激期间BSR降低(P<0.05).与AC组或光刺激前1 min比较,A组光刺激期间BSR降低(P<0.05).结论 采用光遗传学技术再次证实了orexin能神经元可通过PVT发挥全麻促觉醒效应.%Objective To evaluate whether the thalamic paraventricular nucleus mediates orexiner-gic ( orexin ) neurons-induced promotion of emergence from general anesthesia by using the optogenetics method in mice. Methods Twenty healthy male Hcrt-cre mice, aged 8-10 weeks, weighing 20-25 g, were divided into 4 groups ( n=5 each) using a random number table method: retrograde labeled viruses channelrhodopsin group ( R group) , anterograde labeled viruses channelrhodopsin group ( A group) , retro-grade labeled viruses control group ( RC group ) , and anterograde labeled viruses control group ( AC group) . The optogenetics technique was used in each group. Anesthesia was induced and maintained through inhaling 1％ isoflurane and pure oxygen 1. 0 L∕min. Electroencephalogram was monitored througout the procedure with the PowerLab monitoring system. The burst suppression ratio ( BSR) was recorded at 1 min before light stimulation and during light stimulation. Results Compared with RC group or the baseline at 1 min before light stimulation, the BSR was significantly decreased during light stimulation in R group ( P<0. 05) . Compared with AC group or the baseline at 1 min before light stimulation, the BSR was signifi-cantly decreased during light stimulation in group A ( P<0. 05) . Conclusion Optogenetics technique ap-plication once again confirms that orexin neurons can promote emergence from general anesthesia through thalamic paraventricular nucleus in mice.