Enzymatic activities in hypothalamus-thalamus of mice exposed to a magnetic field (ELF-MF)

摘要

In this work are determined enzymatic activities of the electron transport chain, which are the support reactions of electron flow giving energy (ATP), maintaining the redox condition in the human body and essential for life. Metal ions, principally iron and copper, are essential for the activity of the electron flow which could be modified by electromagnetic fields. Heavy metals can substitute the essential mineral elements, blocking numerous reactions and inducing toxicity. Male BALB/c mice were exposed from 1 week to 8 months to extremely low frequency-magnetic fields (ELF-MF) of 27.5 Gauss, in a metabolic cage inside the solenoide, and the cage of the control animals placed at one meter distance. Cerebral regions are dissected and freezed under N_2 until they are submitted to analysis. Hypothalamus and thalamus are determined after the exposure to 50 Hz and 2.75 mT during 7, 14, 21 y 28 days in comparison to control mice. Brain regions are homogenized in buffer and centrifuged, the supernatant submitted to analysis for the complex Ⅰ to Ⅴ and enzymatic activities determined by spectrophotometry-Peltier system under optimal conditions of pH and temperature in relation to maximal activity/mgprotein or/synthase activity. The brain structures analyzed till now don't show a dose-time effect relationship, but there appear marked higher individual responses to the activities of cytochrome oxydase in comparison to the control animals, which could show the existence of the hypersensitivity for certain animals.