吗啡条件性位置偏爱大鼠纹状体谷氨酸递质含量和NR2B亚基表达变化

摘要

Objective: To explore the mechanism underlying the opiates psychical dependence by detecting the changes of glu matic acid and NMDA receptor 2B subunit (NR2B) in the striatum at the pre-receptor and receptor levels in conditioned place preference (CPP) rats. Methods: The CPP rat model was duplicated by subcutaneous injection of morphine for 10 days. The glumatic acid (Glu) and NR2B in the striatum were detected by colorimetry and immunohistochemistry respective ly. Results: Compared with the rats injected with saline, the morphine dependent rats were exhibited with higher contents of Glu and NR2B. Conclusion: The up-regulated Glu and NR2B may contribute to the morphine-induced psychical dependence.%目的:观察吗啡条件性位置偏爱(CPP)大鼠纹状体中谷氨酸递质和N-甲基-D天冬氨酸(NMDA)受体NR2B亚基的变化,从受体前和受体层面探讨通过谷氨酸能系统的阿片类精神依赖机制.方法:吗啡剂量递增连续皮下注射10d,建立大鼠吗啡CPP模型,生理盐水组和吗啡模型组各取10只断头处死,取脑纹状体采用谷氨酸试剂盒测定谷氨酸含量；另取6只经灌注固定后,免疫组织化学检测纹状体中NR2B的表达.结果:与生理盐水组比较,模型组大鼠纹状体谷氨酸含量和NR2B表达均增加.结论:纹状体中谷氨酸递质含量及其受体NR2B表达水平上调在一定程度上参与了在吗啡精神依赖的形成.