毛细管电泳中葡萄糖-羟丙基甲基纤维素体系分离脱氧核糖核酸

摘要

Glycerol,mannitol has been used as additives for the separation of deoxyribonucleic acid (DNA)fragments in entangled solution by capillary electrophoresis. Differing from glycerol and mannitol,the structure of glucose is a ring with 5 hydroxyl groups.This structure helps it easily to form complex with boric acid and hydroxypropyl methylcellulose (HPMC).On the other hand,glucose has large space obstacle compared with glycerol and mannitol.So glucose can markedly improve separation of DNA in HPMC solution by capillary electrophoresis compared with other additives.The factors such as boric acid concentration and pH,influencing formation of complex of HPMC and glucose were studied. High concentration of boric acid could improve the DNA separation in glucose-HPMC matrix system. The optimization pH to separate DNA in HPMC-glucose solution by capillary electrophoresis was 8.3.%葡萄糖作为羟丙基甲基纤维素(Hydroxypropylmethyl cellulose, HPMC)筛分体系的添加剂，可以改善该体系在低浓度时分离脱氧核糖核酸(DNA)的能力。研究了硼酸浓度和pH值对葡萄糖-羟丙基甲基纤维素体系分离性能的影响；并将葡萄糖与其它添加剂如甘油、甘露醇对分离的影响作了比较，葡萄糖特有的环状结构使得其对羟丙基甲基纤维素体系分离能力的提高更为显著。