Metronidazole (MNZ) ester was chosen as a dummy template, methacrylic acid as mono-mer, ethylene glycol dimethacrylate as crosslinker and chloroform as porogen to synthesize dummy-template molecularly imprinted polymers (MIP) for MNZ. The result of batch rebinding tests showed that imprinting factor of the polymer was 2. 02, indicating high recognition ability of the MIP against MNZ. MIP adsorption isotherm was fit to Langmuir adsorption model, therefore it was monolayer adsorp-tion, with maximum binding capacity Qmax of 116 μmol/g and binding constant K of 0. 65 L/mmol. The recoveries of MNZ on MIP and nonimprinted cartridges were 97. 4% and 44. 3% respectively in solid phase extraction (SPE) experiment. However, the analogues of MNZ, MNZ ester and 4-nitrophenol, had recoveries less than 11. 6% on both MIP and NIP in SPE, showing the high selectivity of the dummy template MIP against MNZ. The MIP was successfully used for the separation and concentration of MNZ spiked serum sample with mean recovery of 94. 5%, which demonstrated that MIP was feasible of pre-treatment of MNZ serum sample without template bleeding.%以甲硝唑酯化物(MNZ-Es)为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,氯仿为致孔剂,合成了对甲硝唑(MNZ)具有特异吸附性能的分子印迹聚合物(MIP).由静态吸附实验结果得知,印迹指数为2.02,说明MIP对MNZ具有良好的识别性能.MIP吸附属于Langmuir吸附模型,其对MNZ的吸附属于单层分子吸附,其最大表观吸附量Qmax为116 μmol/g,结合常数K为0.65 L/mmol.在固相萃取实验中,MNZ在分子印迹固相萃取(MISPE)和非印迹固相萃取(NISPE)上的回收率分别为97.4％和44.3％,而结构类似物MNZ-Es和对硝基苯酚在印迹和非印迹柱子上的回收率均小于11.6％,表明替代模板MIP对MNZ具有很高的选择性吸附.应用MISPE分离富集血清样品中的MNZ回收率为94.5％,说明替代模板MIP可实现血清样品中MNZ的选择性分离富集,且没有模板渗漏.
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