高效液相色谱与液相色谱-质谱分析冬虫夏草相关产品之麦角固醇与其衍生物

摘要

Current research uses HPLC and LC-MS to investigate ergosterol and its derivatives contained in a Cordyceps sinensis (Cs) fruiting body obtained from Tibet, an anamorphic Hirsutella sinensis mycelium (Hsm) strain RS6-3 isolated from Cs by our laboratory, and some Cs-associated products, such as Cordyceps militaris (Cm) and Paecilomyces hepialid (Cs-4). Results indicated that the ergosterol in the Cs stroma was approx. 0. 92 g/L, or about 3 times higher than that found in the sclerotium. The Cs stroma contained 2 kinds of Hl-A derivatives, while the Hsm RS6-3 contained 5 kinds of Hl-A derivatives, which were different from those found in the Cs sclerotium. No Hl-A derivative was found in Cs sclerotium, Cm or Cs-4. The results of this investigation may provide a technical foundation for assessing the quality of Cs-associated products.%采用HPLC与LC-MS分析西藏冬虫夏草(Cord yceps sinensis,Cs)子实体、Cs分离的无性世代中国被毛孢(Hirsutella sinensis)菌丝体(Hsm)RS6-3、市售蛹虫草(Cordyceps militaris,Cm)和蝙蝠蛾拟青霉(Paecilomyces hepiali,Cs-4)中所含的麦角固醇与其衍生物.结果表明:Cs子实体子座的麦角固醇比菌核高约3倍,含量约为0.92 g/L,且该子座含2种H1-A衍生物；本实验室所分离的Hsm RS6-3含有5种H1-A衍生物；但Cs子实体菌核、Cm和Cs-4均不含有H1-A衍生物.本研究为虫草相关产品的质量检测提供了技术支持.