用于将DNA疫苗在体导入表皮细胞的微弹加速技术

摘要

It is discussed that the development of delivering DNA vaccine into ski n in vivo, presented a new accelerating method based on two-stage injector . By numerical simulation, flow parameters of injector in different conditions includ ing Mach number, total pressure on the outlet section and negative pressure was got. By experiment, total pressure, negative pressure and speed of particles wer e measured, and distribution of particles was also observed. The numerical simul ation results are coincided with experimental data, which demonstrates that the numerical simulation can serve as guidance for optimal design and operation in p ractice. Particles distributed uniformly, they could be shot into or even pass t he epidermis cells, which can meet the requirement of gene immunity.%分析了将裹着DNA疫苗的微弹射入表皮细胞技术的现状，提出了基于两阶段引射器加 速微弹的方法，进行了数值模拟，得到了不同工况下第二级喷嘴的马赫数等值线、出口截面 上的静压、轴线上的速度以及在负压孔产生的负压等分布。在此基础上，进行了实验研究， 测得了外流场的压力、微弹速度和负压孔的负压值。同时，还观察了钨微弹的实际分布，并 以钨粉为微弹射击鸡皮。结果表明，模拟数据与测量结果一致性好，数值模拟可以指导仪器 设计和实际操作；微弹分布均匀，能射入表皮细胞，还可穿透表皮，能满足基因免疫的要求 。