目的 观察多不饱和脂肪酸对非酒精性脂肪性肝炎(NASH)大鼠肝组织中瘦素、抵抗素和脂联素的影响.方法 将44只大鼠随机分为4组,对照组(10只)给予普通饲料喂养,模型组(10只)、低剂量组(12只)和高剂量组(12只)给予高脂饲料喂养制备NASH模型.低剂量组和高剂量组在喂养8周后加用ω-3多不饱和脂肪酸干预(0.5 g/d,1.0 g/d),12周后处死大鼠.比较各组大鼠体质量、肝质量及肝组织学改变,检测肝组织高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、甘油三酯(TG)及总胆固醇(TC)水平,采用ELISA法和实时荧光PCR方法检测脂肪细胞因子的表达变化.结果 12周后模型组大鼠肝组织病理学检查显示明显脂肪变,体质量、肝质量和各项生化指标均显著高于对照组；与模型组相比,低剂量组和高剂量组体质量和生化指标均有改善且呈剂量相关,其中高剂量组HDL、LDL和TC下降差异有统计学意义( P＜0.05,P＜0.01,P＜0.01)；多不饱和脂肪酸治疗后大鼠肝脏中瘦素、抵抗素显著下降(P＜0.01),而脂联素呈升高倾向,并与剂量呈正比,高剂量组肝匀浆中脂肪细胞因子水平改变与低剂量组差异有统计学意义(P＜0.05),其中抵抗素和脂联素mRNA表达差异有统计学意义(P＜0.01).结论 多不饱和脂肪酸具有改善NASH大鼠脂代谢及脂肪细胞因子表达的作用.%Objective To observe the efficacy of polyunsaturated fatty acid(PUFA) on adipocytokines in rats with NASH. Methods Forty-four rats were randomly divided into 4 groups (control/ NASH/ lower therapy/ higher therapy group). Rats in control group were fed with normal diet,and the other three with high-fat diet. Ω-3 unsaturated fatty acid was added to therapy group from 8th week to 12th week(0. 5 g/d and 1. 0 g/d). The body/liver weight, HDL, LDL, TC and TG in liver of rats in each group were measured. Adipocytokines were detected by ELISA and real time flourescence quantitive PCR. Results NASH model was established successfully. The weight, HDL, LDL, TC and TG were decreased in lower/ higher therapy group, as compared with those in rats with NASH. Changes of HDL, LDL and TC showed significant difference in higher therapy group(P＜0. 05,P＜0. 01 , P＜0. 01). Lower/higher therapy group showed lower leptin, resistin and higher adiponectin levels than those in NASH group. Changes depended on dosage of PUFA. Adipocytokines in liver differed between higher group and lower one (P＜0. 05),among which resistin and adiponectin mRNA expression was of statistical significance ( P＜0. 01). Conclusion PUFA could improve lipid metabolism and regulate the expression of adipocytokines on NASH rats.
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