胶体金免疫层析法检测心肌标志物在诊断急性心肌梗死中的应用

摘要

目的 评价胶体金免疫层析法检测心肌标志物血清肌钙蛋白Ⅰ/肌酸激酶同工酶(CK-MB)/肌红蛋白诊断急性心肌梗死(AMI)的效能.方法 采用双盲、自身对照的试验设计方法,分别采用科卫公司研制生产的肌钙蛋白Ⅰ/CK-MB/肌红蛋白三合一诊断试剂盒和艾康公司生产的试剂盒对140例AMI患者的血液标本进行检测.结果 两种试剂检测肌钙蛋白Ⅰ、肌红蛋白和CK-MB的结果间差异无统计学意义( P ＞0.05).试验试剂检测肌钙蛋白Ⅰ、肌红蛋白、CK-MB的相对敏感度分别为98.4%、99.2%和97.6%,特异度分别为94.4%、90.9%和94.1%.结论 应用胶体金免疫层析法检测肌钙蛋白Ⅰ、CK-MB、肌红蛋白适于AMI的辅助诊断.%Objective To evaluate the efficacy of colloidal gold immunochromatography assay for serum cardiac markers troponin I ( cTnl ) / creatine kinase ( CK - MB ) / myoglobin ( Myo ) in the diagnosis of acute myocardial infarction.Methods Troponin I ( cTnl ), creatine kinase ( CK - MB ) and myoglobin of 140 confirmed patients were tested by cardiac markers produced by Kewei Company and ACON Company in a double - blind, self - controlled trial design.Results There was no significant difference between the two groups in troponin I, myoglobin and CK - MB ( P ＞ 0.05 ).Relative sensitivity of cTnl, myoglobin and CK - MB were 98.4％ , 99.2％ and 97.6％ respectively, and specificity were 94.4％ , 90.9％ and 94.1％ in the trial group.Conclusion Colloidal gold immunochromatography assay of cTnl, CK - MB and Myo is suitable for the diagnosis of acute myocardial infarction.