Objective To explore the influence and mechanism of aspirin on proliferation and migration of aortic vascular smooth muscle cells caused by platelet -derived growth factor BB ( PDGF-BB ) .Methods From August 2014 to September 2015, aortic vascular smooth muscle cell strains (T/G HA-VSMC) were selected and divided into four groups:control group (no other reagent), PDGF-BB group (with 10 ng/ml PDGF-BB), aspirin group (with 5 mmol/L aspirin), and PDGF-BB+aspirin group ( with 10 ng/ml PDGF-BB and 5 mmol/L aspirin ) .OD value of T/G HA-VSMC proliferation at different cultivation times (0, 24, 48, and 72 h) was tested by CCK-8 method, and relative expression quantity of p 21waf1 and p27 kip1 was tested by Western blotting .Cell cycles were tested by flow cytometry , and cell migration was tested by wound -healing assay.Relative expression quantity of matrix metalloproteinase 1 (MMP-1) in four groups was tested by Western blotting. Results When cultivated at 0 h, differences of OD value of T/G HA-VSMC proliferation in four groups showed no statistical significance (P>0.05) .When cultivated at 24, 48, and 72 h, OD value of T/G HA-VSMC proliferation in PDGF-BB group was higher than that in control group (P<0.05) .In aspirin group, OD value of T/G HA-VSMC proliferation was lower than that in control group (P<0.05); when cultivated at 48 and 72 h, OD value of T/G HA-VSMC proliferation in PDGF-BB+aspirin group was lower than that in control group ( P<0.05 ); when cultivated at 24, 48, and 72 h, OD value of T/G HA-VSMC proliferation in aspirin group and PDGF-BB+aspirin group was lower than that in PDGF-BB group ( P<0.05 );when cultivated at 24, 48, and 72 h, OD value of T/G HA-VSMC proliferation in PDGF-BB+aspirin group was higher than that in aspirin group (P<0.05) .Relative expression quantities of p21waf1 and p27kip1 in PDGF-BB group at 24 and 48 h were higher than those at 0 h (P<0.05); when cultivated at 72 h, relative expression quantities of p21waf1 and p27kip1 were lower than those at 0, 24, and 48 h (P<0.05) .When cultivated at 24 h, relative expression quantities of p21waf1 and p27kip1 in PDGF-BB group were lower than those in other three groups (P<0.05) .G0/G1 ratio of PDGF-BB group was lower than that in control group , aspirin group , and PDGF-BB+aspirin group , while ratio of S period was higher than that in other three groups (P<0.05) .Wound-healing assay showed that , when cultivated at 24 h, cell migration rate in PDGF-BB group was lower than that in other three groups (P<0.05) .MMP-1relative expression quantity in PDGF-BB group was higher than that in other three groups (P<0.05); MMP-1relative expression quantitiy in aspirin group and PDGF-BB+aspirin group was lower than that in control group (P<0.05) .Conclusion Aspirin inhibits proliferation and migration of aortic vascular smooth muscle cells caused by PDGF-BB via increasing p21waf1 and p27kip1 and reducing MMP-1 expression.%目的：探讨阿司匹林对由血小板源性生长因子BB ( PDGF-BB ）引起的主动脉血管平滑肌细胞增殖和迁移的影响及其机制。方法2014年8月—2015年9月，选取人主动脉血管平滑肌细胞株( T／G HA-VSMC），分为对照组(不添加任何试剂）、 PDGF-BB组(添加10 ng／ml PDGF-BB ）、阿司匹林组(添加5 mmol／L 阿司匹林）、PDGF-BB＋阿司匹林组(添加10 ng／ml PDGF-BB和5 mmol／L阿司匹林），采用CCK-8法检测4组不同培养时间(0、24、48、72 h） T／G HA-VSMC增殖吸光度(OD值）， Western blotting法检测p21waf1、 p27kip1相对表达量，流式细胞仪检测细胞周期，细胞划痕实验检测细胞迁移率， Western blotting法检测基质金属蛋白酶1(MMP-1）相对表达量。结果培养0 h 时，4组 T／G HA-VSMC增殖 OD 值比较，差异无统计学意义( P ＞0.05）。培养24、48、72 h 时， PDGF-BB组T／G HA-VSMC增殖 OD 值较对照组升高，阿司匹林组 T／G HA-VSMC增殖 OD 值较对照组降低(P＜0.05）；培养48、72 h时， PDGF-BB＋阿司匹林组T／G HA-VSMC增殖OD值较对照组降低(P＜0.05）；培养24、48、72 h时，阿司匹林组和PDGF-BB＋阿司匹林组T／G HA-VSMC增殖OD值较PDGF-BB组降低( P＜0.05）；培养24、48、72 h时， PDGF-BB＋阿司匹林组T／G HA-VSMC增殖OD值较阿司匹林组升高( P＜0.05）。 PDGF-BB组培养24、48 h时p21waf1、 p27kip1相对表达量较培养0 h时升高(P＜0.05）；培养72 h时p21waf1、 p27kip1相对表达量较培养0、24、48 h时降低( P＜0.05）。培养24 h 时， PDGF-BB组 p21waf1、 p27kip1相对表达量较对照组、阿司匹林组和PDGF-BB＋阿司匹林组降低(P＜0.05）。 PDGF-BB组G0／G1期细胞所占比例较对照组、阿司匹林组和PDGF-BB＋阿司匹林组降低， S期细胞所占比例较对照组、阿司匹林组和PDGF-BB＋阿司匹林组升高( P＜0.05）。细胞划痕实验结果显示，培养24 h 时， PDGF-BB组细胞迁移率较对照组、阿司匹林组和PDGF-BB ＋阿司匹林组降低( P＜0.05）。PDGF-BB组MMP-1相对表达量较对照组、阿司匹林组和PDGF-BB ＋阿司匹林组升高( P＜0.05）；阿司匹林组和PDGF-BB＋阿司匹林组MMP-1相对表达量较对照组降低(P＜0.05）。结论阿司匹林通过上调p21waf1、 p27kip1，下调MMP-1而抑制由PDGF-BB引起的主动脉血管平滑肌细胞的增殖和迁移。
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