黄瓜上甜瓜黄斑病毒寿光分离物的初步鉴定及序列分析

摘要

Cucumber leaves infected possibly byMelon yellow spot virus(MYSV) were collected from Shouguang, Shandong Province. These samples were detected by RT-PCR. The amplified products were connected to pEASY -T1 Simple cloning vector and then sequencing was conducted. The results showed that the size of target fragment was 505 bp, which was consistent with the expected result. And homology analysis revealed that the MYSV infected cucumber in Shouguang had closer genetic relationship with MYSV-Sanya isolates (melon -GQ397254) from Sanya,China and MYSV-TW isolates (watermelon -FJ386391) from Taiwan, China. Their homology could reach 98%.%在山东寿光黄瓜保护地种植区采集到疑似感染甜瓜黄斑病毒（Melon yellow spot virus，MYSV）的黄瓜病叶，采用RT-PCR对其进行检测，将扩增产物连接到pEASY -T1 Simple克隆载体后进行测序，结果显示目的片段的大小为505 bp，与预期结果一致；序列同源性比对分析结果表明，黄瓜上MYSV寿光分离物与中国三亚的MYSV-Sanya分离物（甜瓜-GQ397254）和中国台湾MYSV-TW（西瓜-FJ386391）亲缘关系较近，同源性可达到98%。