提高春季西瓜未授粉子房胚状体诱导率的方法

摘要

为提高春季西瓜未授粉子房的胚状体诱导率、加速西瓜单倍体育种进程,以早佳8424西瓜未授粉子房为材料,对影响春季西瓜未授粉子房胚状体诱导率的因素进行了研究.结果表明:摘取子房时尽量保留最长果柄,然后将带果柄子房用0.02 mg·L-1的TDZ溶液浸泡10 min,最后将其置于15 ℃恒温条件下预处理2 d,能够将春季西瓜未授粉子房的胚状体诱导率从3.5%提高到15.9%.%In order to improve embryoid induction rate of watermelon unfertilized ovary and accelerate its haploid breeding process,this experiment took unfertilized ovary of watermelon 'Zaojia8428' as material and studied on the factors affecting induction rate of embryoid grown in spring.The results showed that keeping the fruit stalk as long as possible when the ovary was taken,then steeping it in 0.02 mg·L-1TDZ for 10 min and putting it at 15 ℃ as pretreatment for 2 days could improve the induction rate of embryoid from 3.5% to 15.9%.