Objective :To study how the aerobic exercise of different intensity has therapy effect on nonalcoholic steatohepatitis of rats model .Methods :Randomly divide the fifty Sprague Daw-ley rats into Normal Diets group (ND ) ,High Fat Diet Control group (C ) ,High Fat Diet Low Intensity Exercise group (LE ) ,High Fat Diet Middle Intensity Exercise group (ME ) and High Fat Diet Incremental intensity Exercise group (IE) ,10 rats in each group .The ND group is feed normal diet and the other groups are high fat diet for 16 weeks .Every exercise groups start to run on the animal treadmill since the 11th week during the experiment for 6 weeks .At the end of test ,the rat’s abdominal aortic blood was to measure the level of ALT ,blood fat and adiponectin ,morphology of hepatic tissue was observed ,the level of SOD ,MDA in hepato-cytes was assayed and the mRNA of SIRT1 ,MCP1 ,NCF2 was measured ,and the fat content in liver was observed by PI/OP MRI ,as well as the protein expression of SIRT1 and nuclear NF-κB in hepatocyte .Results :The nonalcoholic steatosis hepatitis and hepatic fibrosis was oc-cured in the C group after 16 weeks high-fat feeding .However ,three types of exercise intensi-ty could ameliorate the lipid deposition and the state of oxidative stress in liver .Compared to the low intensity exercise ,middle and incremental intensity exercise could significantly increase the level of serum HWM adiponectin and the protein expression of SIRT1 in hepatocyte ,su-press the protein expression of NF-κB in nucleus and its downstream such as MCP1 and NCF2mRNA expression in hepatocyte ,so impede the occur of lobuler hepatitis .Conclusion :All three different intensities of aerobic exercise could supress the lipid accumulation in rats liver through weight reduction mechnism .Vigorous aerobic exercise profoundly restrain the transi-tion of NAFLD to NASH of rats through SIRT1 signal transduction axis in hepatocyte ,but low intensity of aerobic exercise couldn’ t .%目的：研究不同强度的有氧运动对 NAFLD大鼠肝脏炎性病变的影响。方法：雄性SD大鼠50只，随机分为普通安静组（ND）、高脂安静组（C）、高脂小强度运动组（LE）、高脂中等强度运动组（M E ）、高脂较大强度运动组（IE ）。普通组和高脂组分别以普通或高脂饲料喂养，共计16周，运动组进行6周跑台运动。实验结束时，进行肝 HE染色组织学分析，PI/PO M RI对肝脂质进行半定量分析，腹主动脉血测定血清肝功能酶和血脂含量，肝组织匀浆测定SOD、MDA水平，荧光定量 PCR和Western Blot ，分别测定SIRT1、MCP1、NCF2、PPARαmRNA表达以及SIRT1、核NF-κBp65蛋白表达。结果：高脂喂养16周后，高脂安静组大鼠发生脂肪肝炎和纤维化变，6周有氧运动可减少运动组大鼠肝脂质沉积，改善肝组织氧化应激；与小强度运动相比，中等及较高强度运动促进 HMW脂联素分泌、增加肝细胞SIRT1基因和蛋白表达，抑制核 NF-κBp65蛋白和减少下游 MCP1、NCF2等炎性细胞因子的表达，显著抑制肝小叶性炎症。结论：不同强度有氧运动可抑制大鼠体重的增加，从而减少因高脂饮食导致的大鼠肝脏脂质沉积；较大强度有氧运动可显著性抑制大鼠 NAFLD向 NASH 方向转化，其机制与促进肝细胞SIRT1信号转导有关，小强度有氧运动抑制大鼠 NASH 效果不显著。
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