OBJECTIVE: To develop a method for the concentration determination of parecoxib in human plasma. METHODS:RP-HPLC method was adopted. The plasma sample was extracted with absolute ether. The determination was performed on Kromasil-C18(250 nm×4.6 nm, 5 μm)column with mobile phase consisted of acetontrile-water (92:8) at the flow rate of 1.0 mL·min-1.Ibuproren (IBF) was used as internal standard and UV detection wavelength was set at 209 nm. Minimum injection interval was 30 min. RESULTS: The linear range of parecoxib was 73.69～442.16 μg·mL-1(r=0.999 2). The method recovery was 97.26％ ～99.24％ and extraction recovery was 79.27％ ～80.51％. The RSD of intra-day and inter-day were less than 2.10％. CONCLUSION: The method is simple, rapid, accurate, reproducible and suitable for the determination of parecoxib concentration in human plasma.%目的:建立测定人血浆中帕瑞昔布的方法.方法:采用反相高效液相色谱法,血浆以无水乙醚提取后进样测定,色谱柱为Kromasil C18,流动相为乙腈-水(92∶8),内标为布洛芬,流速为1.0mL·min-1,检测渡长为209nm,最小进样间隔为30min.结果:帕瑞昔布血药浓度在73.69～442.16 μg·mL-1范围内线性关系良好(r=0.9992);方法回收率为97.26%～99.24%,提取回收率为79.27%～80.51%,日内和日间RSD均≤2.10%.结论:本方法简便、快速、准确、重复性好,可以满足帕瑞昔布血药浓度的测定要求.
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