OBJECTIVE:To study the effects of rAd-p53 injection on the proliferation,apoptosis and autophagy of nephroblas-toma cells. METHODS:Nephroblastoma cells were respectively cultured 20 h with high,medium,low concentration(1×109,1× 108,1×107 VP/mL)of Recombinant human rAd-p53 injection,cells with no injection were the blank control. Cell proliferation,cy-cle,apoptosis and related gene p21,Bax protein expressions were detected,and autophagy gene LC-3,Atg7,Atg12 expressions and number of autophagosomes were also detected. RESULTS:The proliferation inhibition rates of high,medium,low concentra-tion of Recombinant human rAd-p53 injection to nephroblastoma cells were(42.86±3.18)%,(33.64±7.25)%,(16.26±9.07)%;apoptotic rates were (53.85 ± 9.36)%,(37.35 ± 9.64)%,(23.64 ± 10.65)%,respectively. Compared with blank control,cells at period G0/G1 were increased under high,medium,low concentration Recombinant human rAd-p53 injection,effects were relatively obvious under high,medium concentration (P<0.05 or P<0.01);p21,Bax protein and LC-3,Atg7,Atg12 gene expressions were enhanced under high concentration,the number of autophagosomes was increased(P<0.01);Bax protein expression was en-hanced under medium concentration(P<0.05),the other indicators had no obvious changed(P>0.05). CONCLUSIONS:Recom-binant human rAd-p53 injection can inhibit the cell proliferation of nephroblastoma,and induce its apoptosis and autophagy.%目的:研究重组人p53腺病毒注射液对肾母细胞瘤细胞增殖、凋亡及自噬的影响.方法:将肾母细胞瘤细胞分别与高、中、低浓度(1×109、1×108、1×107 VP/mL)的重组人p53腺病毒注射液共同孵育20 h,以不加注射液的细胞为空白对照.检测细胞增殖、周期、凋亡及相关基因p21、Bax蛋白表达,检测细胞中自噬相关基因LC-3、Atg7、Atg12表达和自噬体数量.结果:高、中、低浓度重组人p53腺病毒注射液对肾母细胞瘤细胞的增殖抑制率分别为(42.86±3.18)%、(33.64±7.25)%、(16.26±9.07)%,细胞凋亡率分别为(53.85±9.36)%、(37.35±9.64)%、(23.64±10.65)%.与空白对照比较,高、中、低浓度药物作用下G0/G1期细胞均增加,其中高、中浓度药物效果较明显(P<0.05或P<0.01);高浓度下细胞中p21、Bax蛋白和LC-3、Atg7、Atg12基因表达增强,自噬体数量增加(P<0.01);中浓度下细胞中Bax蛋白表达增强(P<0.05),其余指标无明显变化(P>0.05).结论:重组人p53腺病毒注射液可抑制肾母细胞瘤细胞增殖,诱导细胞凋亡和自噬.
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