HPLC法同时测定祛喘胶囊中木兰脂素、欧前胡素、异欧前胡素的含量

摘要

目的:建立祛喘胶囊中对木兰脂素、欧前胡素和异欧前胡素的HPLC测定方法. 方法: 采用日本资生堂CAPCELL PAK C18色谱柱(150 mm ×4.6 mm,5 μm),以乙腈-水为流动相进行梯度洗脱,柱温为30℃,流速为1.0 ml·min-1,检测波长在0~25 min为278 nm,25~70 min为248 nm,进样量为10 μl. 结果:木兰脂素在6. 126~55. 134 μg·ml-1范围内线性关系良好(r=0.999 7),平均回收率为101.5%,RSD 为1.4%;欧前胡素在9.862 ~88.758 μg·ml-1范围内线性关系良好(r =0. 999 8),平均回收率为99. 6%,RSD为1. 2%;异欧前胡素在4. 830~43. 470 μg·ml-1范围内线性关系良好(r=0. 999 8),平均回收率为100. 2%,RSD为1. 7%. 结论:本法操作简便、结果准确,可用于祛喘胶囊的质量控制.%Objective:To develop a method to simultaneously determine the content of imperatorin, isoimperatorin and magnolin in Quchuan capsules by HPLC. Methods:A Shiseido CAPCELL PAK C18 column(150 mm × 4. 6 mm,5 μm) was used. The mobile phase consisted of acetonitrile(A)-water(B) with gradient elution at a flow rate of 1. 0 ml·min-1 and the column temperature was at 30℃. The detection wavelength was set at 278 nm from 0 to 25 min and 248 nm from 25 to 70 min. The injection volume was 10 μl. Results:The linear range of imperatorin,isoimperatorin and magnolin was 6. 126-55. 134 μg·ml-1(r=0. 999 7), 9. 862-88. 758 μg ·ml-1(r =0.999 8) and 4.830-43.470 μg·ml-1(r =0.999 8), respectively. The average recovery was 101.5% (RSD =1. 4%), 99. 6%(RSD=1. 2%) and 100. 2% (RSD=1. 7%), respectively. Conclusion: The method is convenient, sensitive and accurate, which can be used for the quality control of Quchuan capsules.