目的：探讨表没食子儿茶素没食子酸酯( epigallocatechin－3－ gallate，EGCG )抑制食管癌细胞Ec9706增殖作用及其相关机制。方法流式细胞术检测38例原发性食管鳞癌患者和20例患者正常食管黏膜组织的细胞周期及CDC25A蛋白表达水平。不同质量浓度EGCG (0，100，200，300 mg/L )作用Ec9706细胞24，48 h后流式细胞术检测细胞增殖及CDC25A蛋白表达水平。结果食管癌组织增殖指数为(24.92±11.01)%，显著高于正常食管组织的(14.49±3.83)%（ P＜0.05）；食管癌细胞中CDC25A蛋白表达水平显著高于食管正常组织中的表达水平（ P＜0.05）；不同质量浓度EGCG作用Ec9706细胞24 h后，细胞增殖指数及CDC25A蛋白表达水平显著降低（ P＜0.05）。结论 CDC25A在食管癌组织中高表达，且参与了食管癌组织高增殖状态的形成，EGCG可通过下调CDC25A表达，抑制食管癌细胞增殖。%Objective To explore the effect and mechanism of epigallocatechin-3-gallate ( EGCG ) in inhibiting the proliferation action of esophageal carcinoma cells Ec9706. Methods The expression level of CDC25A protein and cell cycle were detected by the flow cy-tometry in 38 cases of esophageal squamous cell carcinoma and 20 cases of normal esophageal tissue. At 24, 48 h after treating the Ec9706 cells by different concentrations of EGCG ( 0, 100, 200, 300mg/L ) , the expression level of CDC25A and cell cycle were detect-ed by the flow cytometry. Results The proliferation index ( PI ) in the esophageal cancer tissue was ( 24. 92 ± 11. 01 ) %, which was significantly higher than ( 14. 49 ± 3. 83 )% in the normal esophageal tissue, the difference was statistically significant ( P < 0. 05 ) . The expression level of CDC25A protein in the esophageal carcinoma cells was significantly higher than that in the normal esophageal tissue with statistical difference between them ( P < 0. 05 ) . After 24 h different concentrations of EGCG acting on Ec9706 cells, PI and CDC25A protein expression level were decreased significantly, the difference was statistically significant ( P < 0. 05 ) . Conclusion The high expression of CDC25A in esophageal cancer tissue could participate in the formation of high proliferation status of esophageal can-cer tissue. EGCG could inhibit the proliferation of esophageal carcinoma cells by down-regulating the expression of CDC25A.
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