目的：建立测定血安胶囊中没食子酸含量的反相高效液相色谱法。方法色谱柱采用 Hypersil ODS2柱(200 mm ×4.6 mm，5μm)，流动相为甲醇－0.1%磷酸溶液（3：97），检测波长为214 nm，流速为1.0 mL / min。结果没食子酸进样量在0.0211~0.211μg 范围内与峰面积呈良好线性关系（ R2=1），平均回收率为99.28%，RSD =1.73%( n =6)。结论该方法简便、快速、结果准确、重复性好，可用于血安胶囊中没食子酸含量的测定。%Objective To establish a RT - HPLC method for determining the gallic acid content in Xuean Capsule by RP - HPLC. Methods The octadecyl silane bonded silica gel column ODS - C18 was adopted with the mixture solvent of methanol - 0. 1% phospho-ric acid (3 : 97) as the mobile phase, the flow rate was 1. 0 mL / min and the detecting wavelength was 214 nm. Results The sample size of gallic acid in the range of 0. 021 1 - 0. 211 μg / mL showed the good linear relation with the peak area. The average recovery rate was 99. 28% , RSD = 1. 73% ( n = 6 ) . Conclusion It is concluded that with the parameter set used in this study, the gallic acid content in Xuean capsules can be determined in a fast and accurate manner with a good reproducibility.
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