大七厘散中大黄及当归薄层色谱鉴别方法改进

摘要

Objective To improve the TLC identification of Rheum Palmatum and Radix Angelicae Sinensis of Daqili San. Methods Silica gel H with sodium carboxymethylcellulose as adhesive was used as stationary phase,with normal hexane-ethyl acetate-formic acid (30 :10 :0. 5)as developing solvent to separate 5 constituents of Rheum Palmatum in TLC. Hexane-ethyl acetate(4:1)was used as expansion agents in TLC of Radix Angelicae Sinensis. Results The chromatographic picture was clear,distinct,easy to identify and no interference after improvement. Conclusion The improvement of TLC identification of Rheum Palmatum and Radix Angelicae Sinensis in Daqili San is more adaptable and controllable,it can reach the quality control requirements,which is worthy of promotion.%目的 对大七厘散质量标准中的大黄、当归的薄层色谱鉴别法进行改进.方法 在大黄的薄层色谱鉴别条件中,选用羧甲基纤维素钠为黏合剂硅胶H薄层板,以正己烷-乙酸乙酯-甲酸(30:10:0.5)为展开剂,分离大黄含有的5种有效成分;在当归薄层色谱条件的选择上,改用正己烷-乙酸乙酯(4:1)为展开剂.结果 改进后能得到斑点清晰、分离度好的薄层色谱图,且易观察,无干扰.结论 改进后的薄层色谱鉴别方法更适合于大七厘散中大黄、当归的鉴别,符合质量控制要求,值得关注并推广.