The nutrients promoting the production of γ-linolenic acid by Mortierella isabellina were found by studying on metabolic regulation of production process of γ -linolenic acid. The medium nutrients were screened by Plackett-Burman experiment. The results showed that the key factors influencing oil yield were glucose,KNO3 ,sodium acetate and sodium citrate. The four key factors were optimized by re-sponse surface methodology with yield of γ-linolenic acid as response value. Combining Plackett-Bur-man experiment with response surface methodology, the optimal medium formula were obtained as fol-lows:glucose 100. 90 g/L,KNO3 1. 57 g/L,sodium acetate 3. 42 g/L,sodium citrate 4. 56 g/L,yeast ex-tract 3. 75 g/L,KH2PO4 2. 25 g/L,MgSO4·7H2O 0. 60 g/L,CaCl2 0. 30 g/L,FeSO40. 15 g/L,ZnSO4 0. 20 g/L. Under the optimal conditions,the yield of γ-linolenic acid reached 1 525. 20 mg/L,increas-ing by 1. 1 times than the initial yield (725. 90 mg/L) .%研究深黄被孢霉产γ-亚麻酸过程的代谢调控,从中得出对深黄被孢霉产γ-亚麻酸有促进作用的营养成分,并利用Plackett-Burman实验对培养基营养成分进行筛选. 得出葡萄糖、KNO3、乙酸钠、柠檬酸钠为影响产油量的关键因素. 在此基础上,以γ-亚麻酸产量为响应值,采用响应面法优化,确定4个关键因素的最佳水平. 综合Plackett-Burman实验和响应面法确定的最佳培养基配方为:葡萄糖100. 90 g/L、KNO31. 57 g/L、乙酸钠3. 42 g/L、柠檬酸钠4. 56 g/L、酵母膏3. 75 g/L、KH2PO42.25 g/L、MgSO4·7H2O 0.60 g/L、CaCl2 0.30 g/L、FeSO40.15 g/L、ZnSO40.20 g/L.在最佳条件下γ-亚麻酸的产量达1 525. 20 mg/L,比初始产量725. 90 mg/L提高了1. 1倍.
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