甘油二酯异构体高效液相色谱分析法的建立

摘要

A HPLC method was developed for the separation of 1-laurin-3-palmitin diacylglycerol and 1-laurin-2-palmitin diacylglycerol.The optimal chromatographic analysis conditions were obtained as follows:evaporative light scattering detection (ELSD) as detector (drift tube temperature 90 ℃ and N2 flow rate 2.2 L/min),mobile phase of acetonitrile-isopropanol (volume ratio 95 ∶ 5),flow rate 1.0mL/min,column temperature 35 ℃.Under the optimal chromatographic analysis conditions,the standard curve equation of 1-laurin-3-palmitin diacylglycerol (mass concentration range 0.05-5.00mg/mL) was Y =1.832X + 4.564 with a good linear regression coefficient (R2 =0.998 8).The method showed good reproducibility,precision and accuracy,which was beneficial for the qualitative and quantitative analysis of diacylglycerol positional isomers in structural lipids.%建立了1-月桂酸-3-棕榈酸甘油二酯和1-月桂酸-2-棕榈酸甘油二酯两种位置异构体的高效液相色谱分析方法.最佳色谱分析条件为蒸发光散射检测器(漂移管温度90℃,N2流速2.2 L/min),流动相为乙腈-异丙醇(体积比95:5),流速1.0 mL/min,柱温35℃;最佳色谱条件下,测得0.05 ～5.00 mg/mL质量浓度范围内,1-月桂酸-3-棕榈酸甘油二酯的标准曲线方程为Y=1.832X+ 4.564,线性回归系数R2=0.998 8,线性关系良好;该方法具有良好的精密度、准确度与重现性,适合结构脂质等样品中甘油二酯位置异构体定性、定量分析的要求.