参附注射液对大鼠重症急性胰腺炎肾损伤保护作用的实验研究

摘要

Objective To investigate the protective effects of ShenFu injection on kidney of experimental acute pancreatitis in mrs. Methods Twenty and one Wister rats were randomized into the sham-operated group,AP group and Shenfu group. The plasma level of MDA,MPO,SOD,TNF-α and the kidney level of MDA、ON、SOD and historical scores was determined. Results The values of plasma SOD、MDA、MPO、TNF-α and the kidney of MDA、ON and historical scores in Shenfu-gronp were higher than that in the sham-operated group but lower than that in AP group. The values of SOD of plasma and kidney in Shenfu group is higher than that in AP but lower than that in the sharm-operated group. Coneluion Shenfu injection can protect the early injure of kidney of severe acute panereatitis,which mechanism may be to improve the kidney microcirculation,to reduce the production of TNF-α,to decrease the inflammation of plasma and kidney and to decrease the injure of oxygen free radicals.%目的 通过十二指肠闭襻法建立大鼠重症急性胰腺炎模型,参附注射液腹腔注射,研究参附注射液对急性胰腺炎时肾损伤的保护作用及其分子机制.方法 雄性Wister大鼠21只,随机分为对照组、急性胰腺炎组及参附组;检测血浆SOD、MDA、MPO、TNF-α及肾组织SOD、MDA、NO的OD值及病理学评分;扫描电镜观察肾组织超微结构改变.结果 急性胰腺炎组血浆MPO、MDA、NO、TNF-α和肾组织MDA、NO及病理学变化明显高于对照组,同时也高于参附组;但SOD低于对照组和参附组,三组间均有明显差异.结论 参附注射液对重症急性胰腺炎大鼠肾损伤有保护作用,可能机制是改善肾组织微循环,减少TNF-α的生成,减轻血浆及肾组织的炎症反应及减轻氧自由基导致的损伤.