杭州地区乙型肝炎病毒基因分型与临床指标关系研究

摘要

目的：分析杭州地区乙肝病毒基因分型分布情况及与乙肝病毒DNA定量、乙肝两对半定性/半定量、AFP定量各项指标间的联系。方法对临床146例乙肝病毒DNA阳性患者，用PCR法进行乙肝病毒基因分型及乙肝病毒DNA定量检测；用ELISA法进行乙型肝炎两对半定量检测；用夹心法进行AFP定量检测，以分析彼此间的相关性。结果经统计，B型75例，占51.4%；C型62例，占42.5%；非B非C型共9例，占6.2%；男女性患者基因分型的构成比差异无统计学意义。 C型乙肝病毒DNA水平明显高于B型，C型乙肝表面抗原及E抗原定量测定值高于B型。C型患者AFP定量测定均值较B型高，但二者差异无统计学意义(P>0.05)。结论杭州地区乙肝病毒基因型以B型为主，C型次之；C型乙肝病毒DNA含量较B型高，肝脏损害较B型严重。%Objective To study the distribution of HBV genotypes and the relationship between HBV genotypes and the clinical characteristics in Hangzhou area. Methods The genotype, content of HBV-DNA, serological indicators and AFP were detected by RT-PCR, time resolved immunofluorescence method respectively in 146 cases of HBV-DNA positive patients. The relationship between HBV genotype and the clinical characteristics were analyzed by the statistical analysis.Results In the HBV genotyping, there were 75 cases of type B (51.4%),62 cases of type C (42.5%),9 cases of no B or no C type (6.2%);In type C, the HBV-DNA, HbsAg and HbeAg content were significantly higher than the type B.The content of AFP in type C was higher than type B, but with no statistical difference(P>0.05). Conclusion In Hangzhou area, the priority genotype of HBV is type B. Genotype has a certain relationship with the clinical character-istics and the severity of the disease.