汉防己甲素逆转人肝癌耐药细胞株多药耐药性的研究

摘要

Objective To investigate reversal effect on Hep-3B/ADM by TTD and its mechanism.Methods An adriamycin-resistant human HCC cell line(Hep-3 B/ADM)was established.through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Flow cytometry(FCM)was performed to assess the expression of P-170 and the fluorescene intensity of Rh13 in the cells.Results TTD(0.10-1.00 μmoL/L)can reverse multidrug-resistant of Hep-3B/ADM.TTD decreased the expression of P-170 in the cells significantly.Conclusion TTD significantly enhancs ADM-induced cytotoxicity.The mechanism is mediated by inhibiting muhidrug-resistant(MDR1).%目的 研究汉防己甲素对人肝癌多药耐药细胞株Hep-3B/ADM耐药性的逆转作用及其机制.方法 通过阿霉素(ADM)浓度梯度递增诱导法,建立人肝癌多药耐药细胞株Hep-3B/ADM.MTT法检测细胞对化学疗法药物的敏感性;流式细胞仪检测细胞表面多药耐药基因表达产物P-170及分析细胞内若丹明123染剂相对荧光强度.结果 汉防己甲素(0.10～1.00 μmol/L)可逆转肝癌耐药细胞的耐药性;汉防已甲素明显降低细胞表面P-170的表达.结论 汉防己甲素具有增强阿霉素对Hep-3B/ADM细胞的毒性作用,其作用机制与逆转多药耐药基因有关.