The experimental investigation of SBHL on reversing acquired multi-drug resistance on S180 mouse tumor bearing model

摘要

Observe the influence of solasodine hydrochloride (SBHL) on mouse S180's tumor model's acquired multi-drug resistance by measuring the tumor chemo sensitivity, tumor cell apoptosis and gene expression level of P-glycoprotein(P-gp), Lung Cancer Resistance Protein(LRP) and Topoisomerase II. Methods Establish the Multi-drug Resistance (MDR) mouse S180 tumor model by low dose chemotherapy. Treat the mouse S180 tumor model with standard chemotherapy drug CTX and 5-FU with or without SBHL for 4 weeks respectively. At the end of study, the size of tumor was measured and the growth of inhibition was calculated. Tumor cells were isolated from each group then stained with PI followed by flow cytometry. The percentage of apoptosis was calculated based on the PI positive cells vs total cells. The expression of P-gp, LRP and TOPOII in each group was compared by flow cytomery as well. Results The combination treatment of chemotherapy and SBHL significantly increased the inhibition of tumor growth. The SBHL combination treatment also significantly induced tumor cell apoptosis compared to control group. Further study demonstrated that the SBHL combination treatment reduced the expression of P-gp, LRP and TOPOII. Conclusion SBHL can reverse the S180 mouse tumor model's acquired multi-drug resistance by increase tumor chemo sensitivity. The mechanism of this reversal maybe related to the reduction of P-gp, LRP and TOPOII expression.