半边旗5F对系统性硬皮病患者成纤维细胞半胱氨酸天冬氨酸蛋白酶3活性及其酶原活化的影响

摘要

Objective To study the effects of Peris semipinnata 5F on Caspase-3 the enzyme apoptosis correlated of systemic sclerodermaskin fibroblasts and to explore the cause of apoptotic induction of Pteris Semipinnata 5F on Skin Fibroblasts. Methods Change of enzyme activity and protein expression of Caspase-3 was detected by photometric determination and Western blot. Results The result showed that 5F increased the level of Caspase-3 enzyme activity and reduced protein expression with drug concentration increasing. Conclusion 5F increases the level of Caspase-3 enzyme activity and reduces the proenzyme protein expression, which is one mechanism of 5F's inducing the apoptotic of skin fibroblasts.%目的 探讨半边旗5F对系统性硬皮病成纤维细胞半胱氨酸天冬氨酸蛋白酶3(caspase3)活性及其酶原活化的影响,进而了解该酶诱导系统性硬发病患者皮肤成纤维细胞凋亡的机制.方法 用光度检测法和蛋白质印迹法测定半边旗5F对caspase3的酶活性及酶原蛋白表达量的变化.结果 分别用20、40、80μg/ml的半边旗5F 24 h后,Caspase3酶活性分别为(145.5±4.1)%、(276.5±7.5)%、(423.9±14.3)%,作用48 h后各浓度分别为(171.9±13.5)%、(286.7±7.3)%、(444.9±5.5)%,在半边旗5F作用下,Caspase3的酶活性增高,酶原蛋白表达量减少,活化增加.这些变化在一定范围内随着药物浓度的增高和作用时间的延长而增强.结论 半边旗5F通过影响Caspase3的酶原蛋白表达量而增高酶的活性,是诱导系统性硬皮病患者皮肤成纤维细胞的凋亡机制之一.