YEAST HYBRID VECTOR COMPRISING THE YEAST PHO5 PROMOTER,A DNA SEQUENCE ENCODING A SIGNAL PEPTIDE,THE HUMAN TISSUE PLAMINOGEN ACTIVATOR CODING REGION AND A DNA SEQUENCE ENCODING YEAST TRANSCRIPTION TERMINATION SIGNALS,A METHOD FOR PRODUCING THE VECTOR,A YEAST HOST TRANSFORMED BY THE VECTOR,A METHOD FOR PRODUCING THE TRANSFORMED HOST,A METHOD FOR PRODUCING BIOLOGICALLY ACTIVE HUMAN TISSUE PLASMINOGEN ACTIVATOR USING A SO TRANSFORMED HOST AND HUMAN TISSUE PLASMINOGEN ACTIVATOR PRODUCED BY THE METHOD

摘要

The invention relates to yeast hybrid vectors comprising a yeast promoter and a tissue plasminogen activator coding region which is controlled by said promoter, to yeast cells transformed with said hybrid vectors, to yeast cells containing a chromosomally integrated gene for tissue plasminogen activator, and to novel tissue plasminogen activators. The invention also provides processes for the production of said hybrid vectors, of said transformed yeast cells and of tissue plasminogen activators.