转化生长因子β1联合生长分化因子-5体外诱导骨髓间质干细胞向类髓核细胞分化的实验研究

摘要

Objective To explore the possibility of transforming growthβ1 combined with growth differentiation factor-5 inducing bone marrow mesenchymal stem cells to differentiate into nucleus pulposus-like cells.Methods Bone marrow mesenchymal stem cells were taken from Sprague-Dawley rats and flow cytometer was used to detect the expression of CD105, CD90, CD44, CD29, CD45, CD34 and CD24 of two kinds of stem cells. BMSCs proliferating to the third generation were divided into control group, TGF-β1 group, GDF-5 group and TGF-β1+ GDF-5 group. After 14 days' cultivation of inducing medium of different cytokines, RT-PCR were used to detect the expression of collagen typeⅡ, proteoglycan and SOX-9 genes in every group.Results The CD105, CD90, CD44 and CD29 of two kinds of stem cells had positive expressions while CD45, CD34 and CD24 of two kinds of stem cells had negative expressions. After 14 days' inducing cultivation of bone marrow mesenchymal stem cells, gene expression levels of Collagen typeⅡ, Aggrecan and SOX-9 in TGF-β1 group、GDF-5 group and TGF-β1+ GDF-5 group were significantly increased than those in the control group. The difference was statistically significant (P<0.05). The gene expression level of Collagen type Ⅱ, Aggrecan and SOX-9 in TGF-β1+ GDF-5 group were significantly higher than that in TGF-β1 group and GDF-5 group. The difference was statistically significant (P<0.05).ConclusionTGF-β1 and GDF-5 both have the ability to induce bone marrow mesenchymal stem cells to differentiate into nucleus pulposus-like cells and there is synergistic effect between them. The combined application can better induce BMSCs to differentiate into nucleus pulposus-like cells.%目的：探讨转化生长因子β1联合生长分化因子-5体外诱导骨髓间质干细胞向类髓核细胞分化的可能性。方法取SD大鼠骨髓间质干细胞，流式细胞仪检测两种干细胞CD105、CD90、CD44、CD29、CD45、CD34、CD24的表达。将增殖至第三代的BMSCs分为对照、TGF-β1、GDF-5、TGF-β1+ GDF-5四组，分别以含不同细胞因子诱导液培养14d后，采用RT-PCR检测各组细胞Ⅱ型胶原、蛋白多糖、SOX-9基因的表达。结果两种干细胞CD105、CD90、CD44、CD29表达阳性； CD45、CD34、CD24表达阴性。向类髓核细胞诱导培养14d后，TGF-β1、GDF-5、TGF-β1与GDF-5三组的Collagen type Ⅱ、Aggrecan、SOX-9基因表达水平较对照组均有明显升高，差异有统计学意义（P＜0.05）。联合诱导组经过诱导后的Collagen type Ⅱ、Aggrecan、SOX-9基因表达水平明显高于TGF-β1组及GDF-5组，差异有统计学意义（P＜0.05）。结论 GDF-5与TGF-β1都具有诱导BMSCs向类髓核细胞分化的能力，且二者之间具有协同作用，联合应用可以更好的诱导BMSCs向类髓核细胞分化。