支气管肺泡灌洗液中3种肿瘤标志物的检测在肺癌诊断及病情评估中的价值

摘要

Objective To study the contents of three kinds of tumor marker in bronchoalveolarlavage fluid and its value in diagnosis and assessment oflung cancer. Methods 80 cases of lung cancer patients in our hospital from 2013 May to 2014 May were enrolled in observation group,80 cases of benign lung lesions patients over the same period were enrolled in control group.Bronchoalveolar lavage were collected,mRNA content of tumor marker were detected by fluorescence quantitative PCR method,protein content of tumor marker were detected by Elisa method. Results (1)Cyfra21-1,SCC,TSGF content in bronchoalveolar lavage fluid of different TNM stage were different;as TNM stage increase,Cyfra21-1,SCC,TSGF content in BALF increase,difference had statistical significance(P < 0.05);(2)Cyfra21-1,SCC,TSGF content in serum of TNM Ⅰ stage and TNM Ⅱ stage were no different;Cyfra21-1,SCC,TSGF content in serum of TNM Ⅲ stage and TNM Ⅳ were different,difference had statistical significance(P < 0.05); Conclusion tumor markers content in serum in lung cancer patients increase from TNM III stage,Cyfra21-1,SCC,TSGF content in bronchoalveolar lavage fluid increase from TNM Ⅰ stage,tumor markers content detection in BALF is helpful for early diagnosis of lung cancer.%目的：研究支气管肺泡灌洗液中3种肿瘤标志物的含量对肺癌诊断及病情的评估价值。方法将2013年5月～2014年5月期间在我院就诊的80例肺癌患者纳入研究的观察组，将同期在我院就诊的80例肺部良性病变患者纳入研究的对照组，收集支气管肺泡灌洗液，采用荧光定量 PCR 法检测肿瘤标志物的mRNA 含量、采用 Elisa 法检测肿瘤标志物的蛋白含量。结果（1）TNM 不同分期患者支气管肺泡灌洗液中 Cyfra21-1、SCC、TSGF 的含量均高于对照组，且 TNM 分期越高、肿瘤标志物含量越高，差异有统计学意义（P ＜0.05）；（2）TNM Ⅰ和 TNM Ⅱ期患者血清中 Cyfra21-1、SCC、TSGF 的含量于对照组差异无统计学意义（P ＞0.05），TNM Ⅲ、TNM Ⅳ期患者血清中 Cyfra21-1、SCC、TSGF 的含量均高于对照组，差异有统计学意义（P ＜0.05）。结论肺癌患者血清中肿瘤标志物含量从 TNM Ⅲ期开始升高，支气管肺泡灌洗液中Cyfra21-1、SCC、TSGF 的含量从 TNM Ⅰ开始升高，BALF 中肿瘤标志物含量检测有利于肺癌的早期诊断。