急性心肌梗死患者不同时期外周血LXA4与CD4+CD25+CD127-调节性T细胞水平及相关性研究

摘要

Objective To assay the serum LXA4 and CD4+CD25+CD127-regulatory T cells (Treg) levels of patients with acute myocardial infarction (AMI) in different stages, study the function and mechanism of LXA4 in patients with AMI, discuss its effect in the pathogenesis and prognosis of AMI. Methods Flow cytometry and enzyme-linked immunosor-bent analysis (ELISA) methods were used to detect the level of LXA4 and CD4+CD25+CD127-Treg in 58 patients with AMI (AMI group) at the moment of the admission and 48 hours after admission. At the same time 30 cases of normal controls (control gorup) were detected. Then the results were compared. Results The percentage of CD4+CD25+CD127-Treg cells of CD4+CD25+ T cells in the AMI patients at admission was significantly lower than the percentage of 48 hours after admission and the control group (P<0.05 or P<0.01). The percentage of CD4+CD25+CD127-Treg cells of CD4+CD25+T cells in AMI patients 48 hours after admission was significantly higher than control group (P<0.01). The concentration of LXA4 in the AMI patients at admission was significantly higher than the percentage of 48 hours after admission and the control group (P<0.01 or P<0.05). The concentration of LXA4 in the AMI patients 48 hours after admission was significantly higher than the control group (P< 0.01). There was negative correlation between the con-centration of LXA4 and the percentage of CD4+CD25+CD127- Treg cells (r = -0.738, P= 0.002). Conclusion CD4+CD25+CD127-Treg cells and LXA4 play important roles in immune regulation in the occurrence and the development of AMI patients, and has effect of protection in ischemia-reperfusion injury of myocardial cells.%目的：通过检测急性心肌梗死患者不同时期外周血脂氧素A4（LXA4）与CD4+CD25+CD127-调节性T细胞（Treg）水平，研究LXA4在急性心肌梗死患者中的作用及机制，探讨其在此类疾病发病及预后中的作用。方法采用流式细胞分析法和酶联免疫吸附法（ELISA），检测58例急性心肌梗死患者（AMI组）及30名健康体检者（对照组）入院及48 h后外周血LXA4、CD4+CD25+CD127-Treg细胞水平，并进行相关性分析。结果 AMI患者入院时外周血CD4+CD25+CD127-Treg细胞占CD4+CD25+T细胞的百分率显著低于入院48 h及对照组（P<0.05或P<0.01）；AMI患者入院48 h后的外周血CD4+CD25+CD127-Treg细胞占CD4+CD25+T细胞的百分率显著高于对照组（P<0.01）。 AMI患者入院时血浆LXA4水平显著高于48 h后及对照组（P<0.01或P<0.05）；AMI患者入院48 h后血浆LXA4水平显著高于对照组（P<0.01）；AMI患者血浆LXA4水平与CD4+CD25+CD127-Treg细胞百分率呈显著负相关（r=-0.738，P=0.002）。结论 CD4+CD25+CD127-Treg细胞及LXA4均在急性心肌梗死的发生发展中起到了重要的免疫调节作用，在心肌细胞缺血再灌注损伤中起到了保护细胞的作用。