适用于两种设备的免疫比浊法与化学发光法检测血清铁蛋白的比较研究

摘要

Objective: To compare the difference of turbidimetric immunoassay and chemiluminescence were appropriate for different equipment on the one group serum sample. Methods: Turbidimetric immunoassay and chemiluminescence (turbidimetric immunoassay used fully automatic biochemical analyser Synchron CX4PRO/Beckman, chemiluminescence assay used Beckman DXI800 immunoassay analytic system) were applied to detect 176 serum, respectively. Used independent sample t test, paired compared t test and chi-square test to compare the difference between the two methods and analyzed the accuracy of the two methods. Results:There is significant difference between the two method were appropriate for different equipment, the relative coefficient is 0.585; the sensitivity, positive likelihood ratio, negative likelihood ratio,Youden’s index of turbidimetric immunoassay is more accuracy than chemiluminescence;however, the specificity of chemiluminescence is better than turbidimetric immunoassay. And the kappa is not undesirability. Conclusion: there is significant difference between the two method were appropriate for different equipment, and WHO should draw up the uniform and standard equipment and detection method on measure the serum ferritin.%目的：比较适用于两种设备的比浊法与化学发光法在检测同一组样品中血清蛋白含量的差异。方法：使用全自动生化分析仪Synchron CX4PRO/Beckman与Beckman DXI800免疫分析系统，应用免疫比浊法与化学发光法分别检测176例血清铁蛋白含量。对两组检测结果进行两组均数比较的t检验，配对比较的t检验，对两种方法所判断的铁缺乏情况进行卡方检验，并对两种设备所用的两种方法的准确性进行分析。结果：适用于两种设备的两方法间的差异有统计学意义，两种方法的相关系数仅为0.585；二者判断铁缺乏的差异有统计学意义；比浊法的灵敏度、阳性似然比、阴性似然比和约登指数均较高于化学发光法，而化学发光法的特异度高于比浊法；两方法判断铁缺乏情况的一致性分析Kappa值显示，两种方法的一致性不理想，只具有中度一致性。结论：适用于两种设备的两种方法对同一组样品的检测结果间存在统计学差异，两方法判断铁缺乏情况只具有中度一致性，世界卫生组织(WHO)应对检测血清铁蛋白的设备与方法进行标准化统一。