Procedure covalent attachment of ligands for preparing r immunochemical eactivos For use in homogeneous immunoassays employing microparticles (mps) and its application to the measurement of ferritin and soluble transferrin receptor in serum or plasma sangu

摘要

Procedure covalent attachment of ligands for the preparation of immunological reagents for use in homogeneous immunoassays employing microparticles (MPs) and its application to the measurement of ferritin and soluble transferrin receptor, serum or blood plasma. process comprising a reagent for the measurement of substances in biological samples including the use of microparticles (MPs) coated in aqueous suspension antibody (Ab) by a bridging ligand-counterligand. In this reagent PMs are fixed to its surface a counterligand as avidin or streptavidin and specific antibodies are labeled with a ligand such as biotin. Furthermore, the present invention describes a specific method for covalent attachment of ligands on the Fc portion of antibodies allows a spatial arrangement thereof on very favorable to inmunoagregación PMs. As a result of this spatial arrangement increases the number of active antibodies and therefore the amount of antibody required for the preparation of reagents is reduced. Also, due to this favorable spatial arrangement, unspecific reactions due to the rheumatoid factor are eliminated and the stability of reagents is improved. The invention applies to the evaluation of the status of iron stores by the turbidimetric / nephelometric determination of ferritin and transferrin receptors in serum or blood plasma.