HCV感染者血清及外周血单个核细胞HCV RNA检测及其意义

摘要

探讨同时检测HCV感染者血清及外周血单个核细胞(PBMC)中H CV RNA的意义。方法：应用RT-PCR方法对31例HCV感染者进行血清及PBMC HCV RNA检测。结 果PBMC HCV RNA阳性率(74.19%，23/31)显著高于血清HCV RNA阳性率(23.03%，9/31)。 结论 ：同时检测HCV感染者血清及外周血单个核细胞(PBMC)中HCV RNA可减少漏诊，PBMC中的HCV 可能是HCV感染慢性化的重要原因之一。通过对HCV感染者同时进行血清及PBMC中HCV RNA检 测，可为丙型肝炎的诊断、治疗及预后的判断提供较为可靠的指标，对丙型肝炎慢性化机制 的研究具有重要意义。%To investigate the significance of HCV RNA detection in PBMC in patients with HCV infection.Methods:Al l of 31 sera and PBMC taken from HCV infection were used for HCV RNA detection by RT-PCR.Results:PBMC HCV RNA positive rate (74.19%,23/31)was sig ni ficant higher than those of sera HCV RNA (23.03%,9/31)(χ2＝11.770,P＞0.005).Conclusions:PBMC HCV may be one of important reasons for mechani sm of chronicity pathogenesis HCV infection