RegⅣ、EGFR和Survivin在结直肠腺瘤癌变过程中的表达及意义

摘要

目的 研究再生基因Ⅳ(RegⅣ)、表皮生长因子受体(EGFR)及Survivin在结直肠腺瘤癌变过程中的表达,并分析其与结直肠腺瘤及结直肠癌临床病理特征的关系.方法 采用免疫组织化学结合组织芯片法检测结直肠正常黏膜(150例)、腺瘤(77例)、结直肠癌(150例)RegⅣ、EGFR及Survivin的表达,并分析其与临床病理特征的关系.结果 RegⅣ、EGFR、Survivin在结直肠正常黏膜、腺瘤及癌组织中,表达逐渐升高,差异有统计学意义(P<0.05).RegⅣ、Survivin与结直肠腺瘤大小及分级相关,EGFR仅与腺瘤的分级相关;RegⅣ、EGFR与结直肠癌的分化程度、 淋巴结转移及TNM分期密切相关,Survivin与结直肠癌的淋巴结转移及TNM分期密切相关(P<0.05),而3者与结直肠癌患者的性别、年龄、肿瘤大小及浸润深度无关(P>0.05).结直肠腺瘤组织内RegⅣ、EGFR、Survivin 3者无相关性(P>0.05);结直肠癌组织内RegⅣ与EGFR、RegⅣ与Survivin、EGFR与Survivin表达呈正相关(P<0.05).结论 RegⅣ、EGFR及Survivin在结直肠腺瘤癌变过程中逐渐升高,提示3者共同参与结直肠腺瘤癌变的发生,且与结直肠癌的TNM分期密切相关,联合检测有利于对结直肠癌的预后进行评估,并为研发靶向药物提供理论支持.%Objective To investigate the expressions of regenerating islet-derived family member Ⅳ (RegⅣ), epidermal growth factor receptor (EGFR) and survivin in canceration of colorectal adenomas and analyze the correlations with clinicopathological features. Methods Immunohistochemistry combined with tissue microarray was used to detect the expressions of RegⅣ, EGFR and survivin in normal colorectal mucosa from 150 cases, colorectal carcinomas from 150 cases, and adenomas from 77 cases. The relationships among RegⅣ, EGFR, survivin and clinicopathological features were statistically analyzed. Results The positive rates of RegⅣ, EGFR and survivin in the colorectal adenomas and the colorectal carcinomas were significantly higher than those in the normal mucosa (P<0.05). The expressions of RegⅣ and survivin were positively correlated with adenoma size and degree of dysplasia, while EGFR only correlated with dysplasia grade of adenomas. The expressions of RegⅣ and EGFR in the colorectal carcinomas were significantly correlated with differentiation, lymph node metastasis and TNM stage, while the expression of survivin had positive correlations to lymph node metastasis and TNM stage (P<0.05), but none of them was associated with patients' gender, age, tumor size or depth of invasion (P>0.05). There was no correlation among RegⅣ, EGFR and survivin expressions in the adenomas, while the RegⅣ, EGFR and survivin expressions in the colorectal carcinomas were positively correlated (P<0.05). Conclusions RegⅣ, EGFR and survivin are up-regulated in colorectal adenomas and carcinomas, which indicates that they may play important roles in the canceration of colorectal adenomas and are closely correlated to TNM staging of colorectal carcinomas. Combined detection of the three markers possibly has certain referential value in evaluation of the prognosis of colorectal adenomas and carcinomas, and in developing the target medicine for colorectal carcinomas.