他克莫司联合罗格列酮对TNF-α诱导的HaCaT细胞LL37及NF-κB表达的影响

摘要

目的:确定他克莫司与罗格列酮单独及联合应用对肿瘤坏死因子-α(TNF-α)诱导的HaCaT细胞HL37及核因子- κB(NF-κB)表达的影响.方法:利用TNF-α诱导体外培养的HaCaT细胞处于炎性状态,在不同浓度的他克莫司与罗格列酮单独及联合作用下,采用RT- PCR法检测LL37的表达情况,采用免疫细胞化学(SABC)法检测NF-κB的表达情况.结果:(1)TNF-α诱导的HaCaT细胞中LL37及NF- κB的表达显著高于对照组(P＜0.05).(2)他克莫司、罗格列酮单独及联合应用均可显著抑制TNF-α诱导的HaCaT细胞LL37及NF- κB的表达(P＜0.05)；二者联合应用的作用效果均较单独用药组更强(p＜0.05).结论:他克莫司和罗格列酮联合应用能增强其对TNF-α诱导的LL37及NF-κB表达的抑制作用.%Objective: To determine the effects of tacrolimus and rosiglitazone on the expression of LL37 and nuclear fectar - KB (NF-KB) in TNF-α induced HaCaT cells. Method: HaCaT cells were cultured in vitro, and then stimulated by TNF - α to induce inflammation. After treated with taerolimus and rosiglitazone in different concentration, the expression of LL37 was measured by reverse transcriptase polymerase chain reaction (RT - PCR), and the expression of NF - KB by inimunocytochernigtiy. Results: (1) The expression of LL37 and NF - KB was significantly increased in TNF -α- induced HaCaT cells than in cells without TNF - α induced (P<0.05). (2) Although tacrolimus and rosiglitazone, alone or in combination, inhibited the expression of LL37 and NF - KB in TNF -α induced HaCaT cells, the effect was stronger when treated in combination than each alone (P<0.05). Conclusion: Taerolimus and rosiglitazone can markedly depress the expression of LL37 and NF - KB in TNF - a induced HaCaT cells. The effects were stronger when combination than each alone.