他克莫司联合罗格列酮对TNF -α诱导的HaCaT 细胞增殖分化的影响

摘要

目的:评价他克莫司联合罗格列酮对TNF -α诱导的HaCaT 细胞体外增殖和分化的影响.方法:他克莫司(10μmol/L)和不同浓度罗格列酮单独及联合作用于TNF -α诱导的HaCaT 细胞48 h 后,用CCK-8 法测定细胞增殖,逆转录- 聚合酶链反应(RT-PCR)检测增殖分化标志物角蛋白6 及外 皮蛋白mRNA 的表达水平;结果:他克莫司和罗格列酮均可抑制TNF -α诱导的HaCaT 细胞体外增 殖,下调角蛋白6 mRNA 表达,上调外皮蛋白mRNA 表达,且联合用药组的作用更强.各组细胞生长抑 制率:他克莫司10 μmol/L 组为(21.71 ± 5.36)%,罗格列酮组10、20、40、80 μmol/L 组分别为(5.04 ± 7.12)%、(12.56 ± 6.93)%、(21.41 ± 5.95)%和(39.17 ± 8.03)%,联合1 ～ 4 组分别为(27.60 ± 6.68)%、(38.35 ± 7.24)%、(48.60 ± 8.40)%和(65.21 ± 4.53)%.结论:他克莫司、罗格列酮单独或联合应用 均可抑制TNF -α诱导的HaCaT 细胞体外增殖,促进其分化.%Objective:To determine the effect of combined tacrolimus with rosiglitazone on the proliferation and differentiation of TNF —α induced HaCat cells. Method:After TNF — alpha — induced HaCaT cells were treated with lacrolimus ( 10 μmol/L) and rosiglitazone ( 10. 40 μmol/L) single or in a combined way for 48 hours, the proliferation of the cells were detected by CCK — 8 assay, and the expression of kerain6 and involucrin mRNA were semi — quantitatively analyzed by reverse transcription polymerase chain reaction ( RT — PCR) . Results:After 48 hours, the proliferation inhibition rate of combined group was significantly higher than single groups. Involucrin mRNA expression was significantly up — regulated. keratin6 mRNA expression significantly down — regulated and the combined groups had stronger effect on the expression. Conclusion:Tacrolimus and rosiglilazone can inhibit the proliferation of TNF — alpha — induced HaCaT cells and induce the differentiation in single or combined way.