杞菊滴眼液治疗实验性干眼症泪液减少和角膜上皮病变

摘要

目的 观察杞菊滴眼液对干眼症家兔泪液分泌,及干眼症大鼠角膜修复的影响.方法 泪腺注射阿托品制作干眼症家兔模型.将20只实验家兔分为溶剂对照组、卡波姆滴眼液组、杞菊滴眼液低、中、高剂量组,分别予双眼点杞菊滴眼液的溶剂、0.2%卡波姆滴眼液、杞菊滴眼液(每日2次、4次、8次),测定造模前、第1次泪腺注射阿托品后及治疗第3天、第7天时schirmer试验的值.摘除大鼠双侧泪腺,双眼点1%苯扎溴铵溶液制作角膜上皮损伤干眼症大鼠模型,将40只大鼠分为溶剂对照组、羧甲基纤维素钠滴眼液组、杞菊滴眼液低、中、高剂量组.羧甲基纤维素钠滴眼液组予双眼点0.5%羧甲基纤维素钠滴眼液,其余各组用药情况同家兔,记录治疗第7、14和21天角膜荧光素染色的评分值.结果 家兔治疗第3天,卡波姆滴眼液组与溶剂对照组相比.schirmer试验值增加,差异有统计学意义(P<0.05),治疗第7天,杞菊滴眼液中、高剂量组与溶剂对照组相比,schirmer试验值增加,差异有统计学意义(P<0.05).大鼠治疗14天,杞菊滴眼液低、中、高剂量组的角膜荧光素染色评分低于溶剂对照组(P<0.05),治疗21天,杞菊滴眼液低、中、高剂量组和羧甲基纤维素钠滴眼液组的角膜荧光素染色评分均低于溶剂对照组(P<0.05).结论 杞菊滴眼液可缓解实验性干眼症泪液减少,减轻角膜上皮损伤.%OBJECTIVE To examine the effect of Qiju eye drops on the increasing lacrimal secretion and the repairing of corneal epithelial lesion of dry eye rabbit. METHODS The rabbit dry eye model was prepared by injecting atropine in lacrimal gland. The 20 rabbits were grouped into solution group, caromer eye drop group and Qiju eye drop groups of three different dosages. Solutions, 0.2％ carbomer eye drops and three dosages of Qiju eye drops (2,4 and 8 times per day)were diffused into both eyes. The schirmer values were measured before model preparing,after first injecting atropine and day 3,7 after treatment. The rats corneal epithelial lesion dry eye model was prepared by excising both lacrimal glands and diffusing 1％ benzalkonium bromide solution into eyes. The 40 rats' eyes were grouped into solution group, carboxymethylcellulose sodium eye drop group and three dosages of Qiju eye drop groups. Eye drops of 0.5％ carboxymethylcellulose sodium were diffused into both eyes in carboxymethylcellulose sodium eye drop group and other groups were same with rabbit model. Corneal fluorescence staining scoring values were examined on day 7,14 and 21 after treatment. RESULTS There were significant differences between carbomer eye drop group and solution group on day 3 after treatment(P＜0.05 ).The schirmer value increased in carbomer eye drop group. There were significant differences among high dosage and medium dosage Qiju eye drop groups with solution group on day 7 after treatment (P＜0.05). The schirmer value increased in high dosage and medium dosage Qiju eye drop groups. Corneal fluorescence staining scoring values of three dosages of Qiju eye drop groups were significant less than that of solution groups on day 14 after treatment (P＜0.05). Corneal fluorescence staining scoring values of three dosages of Qiju eye drop groups and carboxymethylcellulose sodium eye drop group were significant less than that of solution group on day 21 after treatment(P＜0.05). CONCLUSIONS Qiju eye drops could buffer low lacrimal secretion and lighten corneal epithelial lesion of experimental dry eye.