枸杞多糖对人视网膜色素上皮细胞脂褐素清除作用的影响

摘要

OBJECTIVE To investigate the effect of lycium barbarum polysaccharide (LBP) to the revesal of cultured human retinal pigment epithelial (RPE)cells lipofuscin accumulation in vitro. METHODS The human RPE cells were fed with porcine photorecepter outer segments (POS) for 3 weeks, to accumulate lipofuscin in RPE cells. The effects of 0~1 000 μg/ml LBP on hRPE lipofuscin were examined by flow cytometry (FCM) at 12, 24, 48 h. RESULTS The fluorescence level of Lipofuscin in groups of 10~1 000 μg/ml lycium barbarum polysaccharide were significantly different statistically from the control group (P<0.01); Group of 1 000 |xg/ml LBP had the minimum fluorescence level. CONCLUSIONS Lycium barbarum polysaccharide could remove hRPE lipofuscin effectively and 1 000 μg/ml LBP has the bigeest proliferation activitivity.%目的 探讨枸杞多糖(lycium barbarum polysaccharide,LBP)在预防或阻止视网膜色素上皮老年性改变中所起的作用.方法 将提取纯化的猪眼光感受器外节(photorecepter ourer segment,POS)与人视网膜色素上皮(retinal pigment epithelium,RPE)细胞共养3周,建立RPE脂褐素模型.将脂褐素化的RPE细胞分为4组,分别加入浓度为0μg/ml(空白对照组)、10 μg/ml、100 μg/ml、1 000μg/ml的LBP培养12h、24 h、48h,流式细胞技术测定不同浓度LBP干预后的人RPE细胞内脂褐素值.结果 各LBP培养组人RPE细胞在不同时间的脂褐素荧光面积均低于空白对照组,差异有统计学意义(P＜0.01)；枸杞多糖浓度为1 000 μg/ml时人RPE细胞内脂褐素荧光面积最小.结论 10 μg/ml、100μg/ml、1 000 μg/ml的LBP具有促进体外培养的人RPE细胞清除脂褐素的作用；浓度为1 000μg/ml时对hRPE细胞内脂褐素的清除作用最强.