煤尘可吸入颗粒物对大鼠支气管肺组织致炎作用的研究

摘要

目的：探讨可吸入颗粒物煤尘对支气管肺组织的相关致炎作用。方法：采集并分析矿井煤尘可吸入颗粒物，用其染毒活体大鼠，然后观察大鼠肺组织的病理变化，并测定大鼠肺泡灌洗液中的白细胞总数和分类计数及肺匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及细胞因子诱导的中性粒细胞趋化因子(CINC)。结果：矿井煤尘组的病理特点以炎症改变为主，并部分显示出过敏性特征。各项观察指标与对照组比较均有显著差异。结论：可吸入颗粒物矿井煤尘可能通过刺激体内炎性细胞募集、大量释放炎症因子，导致局部炎症反应的发生。%Objective:To study the effect of coal dust inhalable particulate mat er on airway inflammation in rats. Methods:Particles at the wel were col ected,analyzed and PM10-treatmodel of rats were establish.Lung tissue histopathology was observed.The counts of total leukocytes and neutrophils in bronchoalveolar lavage fluid(BALF) and superoxide dismutase(SOD), glutathion peroxidase(GSH-Px), malondiadehyde(MDA) cytokin-induced neutrophil chemotactics(CINC) in lung homogenate were measured.Results:The counts of total leukocyt macrophages and neutrophils in PM10-treat group were increased significantly than those in control group (p<0.05). The pathological changes of coal dust group indicated that it could cause airway injury. Conclusion:The mechanism of the airway inflammation by IP is complicated,The terminal way is inflammatory reaction by inflammatory cells and cellfactors.