枸杞类胡萝卜素的分离纯化方法

摘要

本文对枸杞类胡萝卜素的浸提条件进行了研究，得到的最佳参数是：浸提液为石油醚-丙酮（1：3，v/v）混合溶剂，料液比为90(g/mL)，在常温下分3次浸提，总浸提时间为1.5h。枸杞总类胡萝卜素的含量为552.57mg/kg。洗脱剂选择正己烷：丙酮=90：10（v/v），吸附剂选择为氧化镁：硅藻土=1：2（w/w），薄层展开剂选择为石油醚-丙酮=17：4，此条件下对枸杞色素提取液、枸杞总类胡萝卜素样品有很好的分离效果。%In this paper, the carotenoids extraction conditions were studied. We can use petroleum ether and acetone(1:3,v)as extract solvent, and select the solid-liquid ratio of 90 (g/mL). Then we extract lycii carotenoids three times spending 1.5h under this condition. We select hexane acetone(90:10,v)as eluent, magnesium oxide diatomite(1:2,w/w)as adsorbent. Besides, we select petroleum ether and acetone as the developing solvent of TLC. Under this condition, the separation effect for pigment of Fructus Lycii extract and total carotenoid sample is superior.